Green UHPLC Method for Simultaneous Determination of Febuxostat and Diclofenac in Pharmaceutical Dosage Form and Human Plasma  

作　　者：Shimaa A. Mahmoud Amira M. El-Kosasy Fatma A. Fouad Shimaa A. Mahmoud;Amira M. El-Kosasy;Fatma A. Fouad(Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt;Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt)

机构地区：[1]Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt [2]Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt

出　　处：《Journal of Analytical Sciences, Methods and Instrumentation》2021年第4期29-45,共17页分析科学方法和仪器期刊（英文）

摘　　要：Eco-friendly Ultra-high-performance liquid chromatography (UHPLC) with green aqueous-organic mobile phase was applied for the simultaneous determination of febuxostat (FEB) and diclofenac (DIC) with the composition of water:ethanol (85:15<span> </span>v/v) utilizing phenomenex Kinetex C₁₈ column (4.6 × 100 mm 2.6 μm), flow rate 1 ml/min and<b><span> </span></b>UV detection at 280 nm<b><span> </span></b>with linear ranges of 0.4 - 4.0 μg/mL and 0.5 - 5.0 μg/mL for FEB and DIC, respectively. The proposed method was also successfully applied to analyze the two drugs in pharmaceutical dosage form and human plasma. The results obtained were validated and statistically analyzed and found to be in accordance with those given by reported methods. Moreover, the greenness of the developed method is assessed using suitable analytical Eco-Scale and GAPI tools<b><span> </span></b>and comparison with the previously published methods have been carried out to indicate the priority of the proposed method. UHPLC is considered eco-friendly method regarding uses of safe solvents, simple, accurate and short time of analysis.Eco-friendly Ultra-high-performance liquid chromatography (UHPLC) with green aqueous-organic mobile phase was applied for the simultaneous determination of febuxostat (FEB) and diclofenac (DIC) with the composition of water:ethanol (85:15<span> </span>v/v) utilizing phenomenex Kinetex C₁₈ column (4.6 × 100 mm 2.6 μm), flow rate 1 ml/min and<b><span> </span></b>UV detection at 280 nm<b><span> </span></b>with linear ranges of 0.4 - 4.0 μg/mL and 0.5 - 5.0 μg/mL for FEB and DIC, respectively. The proposed method was also successfully applied to analyze the two drugs in pharmaceutical dosage form and human plasma. The results obtained were validated and statistically analyzed and found to be in accordance with those given by reported methods. Moreover, the greenness of the developed method is assessed using suitable analytical Eco-Scale and GAPI tools<b><span> </span></b>and comparison with the previously published methods have been carried out to indicate the priority of the proposed method. UHPLC is considered eco-friendly method regarding uses of safe solvents, simple, accurate and short time of analysis.

关 键 词：FEBUXOSTAT DICLOFENAC Ultra-High-Performance Liquid Chromatography Eco-Scale Green Analytical Procedure Index Tools 

分 类 号：R97[医药卫生—药品]