Molecular basis of scavenging effect of zonisamide on hydroxyl radical <i>in vitro</i>  

作　　者：Ken-ichi Tanaka Takeshi Nanba Tomoyuki Furubayashi Yasuko Noda Luther James Willmore Akitane Mori 

机构地区：[1]Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Shikatacho, Japan [2]Department of Brain Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Shikatacho, Japan [3]Department of Clinical Pharmacy, Shujitsu University School of Pharmacy, Nishigawara, Japan [4]Department of Neurology, Saint Louis University School of Medicine, St. Louis, USA

出　　处：《Journal of Biophysical Chemistry》2012年第3期256-258,共3页生物物理化学（英文）

摘　　要：Zonisamide (ZNS), a commonly used anticonvulsant, scavenged hydroxyl radicals at a clinically relevant concentration. Reactants of ZNS with hydrogen peroxide and with hydrogen peroxide plus UV irradiation, yielding hydroxyl radicals, were analyzed by the LC/MS technique. Many small fragments were found in the both reactions, suggesting that ZNS was decomposed not only by hydroxyl radicals but also by hydrogen peroxide. Furthermore, mass-fragment-grams showed that m/z: 213 (ZNS itself) was decreased markedly and m/z: 118 (may be a decomposed product by ring cleavage of ZNS) was detected specifically by treatment with hydroxyl radical. These data suggested that ZNS may react directly with free radicals.Zonisamide (ZNS), a commonly used anticonvulsant, scavenged hydroxyl radicals at a clinically relevant concentration. Reactants of ZNS with hydrogen peroxide and with hydrogen peroxide plus UV irradiation, yielding hydroxyl radicals, were analyzed by the LC/MS technique. Many small fragments were found in the both reactions, suggesting that ZNS was decomposed not only by hydroxyl radicals but also by hydrogen peroxide. Furthermore, mass-fragment-grams showed that m/z: 213 (ZNS itself) was decreased markedly and m/z: 118 (may be a decomposed product by ring cleavage of ZNS) was detected specifically by treatment with hydroxyl radical. These data suggested that ZNS may react directly with free radicals.

关 键 词：ZONISAMIDE HYDROXYL RADICALS LC/MS ESR 

分 类 号：R73[医药卫生—肿瘤]