国家自然科学基金(30370326)

作品数：3被引量：17H指数：3
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相关期刊：《Acta Biochimica et Biophysica Sinica》更多>>
相关主题：BOMBYX_MORISILKWORMITSGENETEST更多>>
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Structural Analysis of Fibroin Heavy Chain Signal Peptide of Silkworm Bombyx mori被引量：9
《Acta Biochimica et Biophysica Sinica》2006年第7期507-513,共7页Sheng-Peng WANG Ting-Qing GUO Xiu-Yang GUO Jun-Ting HUANG Chang-De LU 
This work was supported by the grants from the National Natural Science Foundation of China （No. 30370326 and No. 30470350）
To study the minimal length required for the secretion of recombinant proteins and silk proteins in posterior silk gland, the signal peptide （SP） of the fibroin heavy chain （FibH） of silkworm Bombyx mori was syste...
关键词：signal peptide fibroin heavy chain silkworm  Bombyx mori rAcMNPV 
Loss of Posterior Silk Gland Transcription Specificity of Fibroin Light Chain Promoter due to Absence of 41 bp Sequence Containing Possible Inhibitor Binding Sites被引量：6
《Acta Biochimica et Biophysica Sinica》2005年第12期819-825, ,共7页Ting-Qing GUO Jian-Yang WANG Sheng-Peng WANG Xiu-Yang GUO Ke-Wei HUANG Jun-Ting HUANG Chang-De LU 
supported by the grants from the National NaturalScience Foundution of China(No.30370326.No.30470350)
The gene encoding fibroin light chain protein （FibL） is specifically expressed in the posterior silk gland of silkworm and repressed in other tissues. The binding sites of several transcription factors involved in t...
关键词：promoter specificity fibroin light chain SILKWORM Bombyx mori recombinant AcMNPV 
Introduction of Foreign Genes into Silkworm Eggs by Electroporation and Its Application in Transgenic Vector Test被引量：7
《Acta Biochimica et Biophysica Sinica》2004年第5期323-330,共8页Xiu-YangGUO LiangDONG Sheng-PengWANG Ting-QingGUO Jian-YangWANG Chang-DeLU 
This work was supported by the grants from the Natural Science Foundation of China (No. 30370326) and the Science and Technology Commit-tee of Shanghai (No. 024319209).
Electroporation as a methodology to introduce foreign genes into silkworm eggs was system- atically analyzed. The foreign gene in both the newly hatched and 3rd instar larva DNA can be detected by PCR. T...
关键词：silkworm eggs gene introduction ELECTROPORATION TRANSPOSON homologous sequences 
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