国家自然科学基金(39870676)

作品数:5被引量:35H指数:3
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相关作者:陈系古钟女奇黄文革陈颖青黄冰更多>>
相关机构:中山医科大学中山大学更多>>
相关期刊:《中国实验动物学报》《Acta Biochimica et Biophysica Sinica》《Progress in Natural Science:Materials International》《中国病理生理杂志》更多>>
相关主题:TETRACYCLINETET-ONES细胞血清培养集落更多>>
相关领域:生物学农业科学医药卫生更多>>
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Tetracycline-inducible Expression Systems:New Strategies and Practices in the Transgenic Mouse Modeling被引量:12
《Acta Biochimica et Biophysica Sinica》2007年第4期235-246,共12页Yan SUN Xigu CHEN Dong XIAO 
supported by the grants from the National Natural Science Foundation of China(No.30271177 and 39870676);the National 9th Five-year Program(No.101033);the Major Science and Technology Projects of Guangdong Province(No.B602);the Natural Science Foundation of Guangdong Province(No.021903);the Postdoctoral Fellowship Foundation of China(Series 29);the Special Fund of Scientific Instrument Collaborative Share-net in Guangzhou(No.2006176)
To accurately analyze the function of transgene(s) of interest in transgenic mice, and to generate credible transgenic animal models for multifarious human diseases to precisely mimic human disease states, it is cri...
关键词:tetracycline-inducible expression system Crellox P system transgenic mouse modeling leaky expression stringent control lineage-specific gene expression lineage-specific RNAi 
Tetracycline-controlled transcriptional regulation systems: countermeasures to eliminate basal transgene leaks in Tet-based systems被引量:2
《Progress in Natural Science:Materials International》2007年第1期11-19,共9页XIAO Dong SUN Yan GU Weiwang CHEN Xigu 
Supported by National Natural Science Foundation of China(Grant Nos.30271177 and 39870676);National 9th Five-Year Plan Program(Grant No.101033);Major Science and Technology Projects of Guangdong Province(Grant Nos.B602 and 2003C60101);Natural Science Foundation of Gdoctoral Fellowship Foundation of China(Series 29)
To analyze the function of any given transgene(s) accurately in transgenic mice, and to produce credible transgenic animal models of various human diseases (precisely and realistically mimicking disease states), i...
关键词:tetracycline inducible system transgenic mice leaky expression transcriptional silencer 
基于四环素调控系统的HD1BEL-7402 Tet-on细胞模型的建立被引量:1
《中国病理生理杂志》2006年第11期2281-2283,共3页钟女奇 杨国柱 陈系古 
"九五"国家重点攻关项目资助(No.96A230602);国家自然科学基金资助项目(No.39870676)
目的:建立表达四环素调控系统(Tet-on)的人肝癌细胞模型(HD1 BEL-7402 Tet-on),为研究四环素调控系统对基因的表达与调控提供一种细胞模型。方法:用四环素调控表达系统(Tet-on),通过脂质体法转染BEL-7402细胞,经G418筛选出高表达Tet-on...
关键词:BEL-7402细胞 转染 基因 TET-ON 模型 动物 
Temporal Control of Cre Recombinase-mediated in Vitro DNA Recombination by Tet-on Gene Expression System被引量:3
《Acta Biochimica et Biophysica Sinica》2005年第2期133-138,共6页Zhong-MinGUO KangXU YingYUE BingHUANG Xin-YanDENG Nü-QiZHONG XunHONG Xi-GuCHEN DongXIAO 
This work was supported by the grants from the National Natural Science Foundation of China(No.30271177 and No.39870676);the Natural Science Foundation of Guangdong Province(No.021903);the Postdoctoral Fellowship Foundation of China(Series 29)
Conditional gene expression and gene deletion are important experimental approaches for examining the functions of particular gene products in mouse models. These strategies exploiting Cre-mediated site-specific DNA r...
关键词:conditional gene regulation Cre recombinase human apolipoprotein E gene (apoE) promoter Tet-on gene expression system universal transgenic system 
小鼠胚胎干细胞在六种培养体系的培养观察被引量:18
《中国实验动物学报》2000年第1期1-6,共6页黄冰 黄文革 钟女奇 陈颖青 陈系古 
国家九五攻关课题! ( 96A2 3 0 60 2 );国家自然科学基金资助! ( 3 9870 676)
目的 观察小鼠胚胎干细胞在六种培养体系中的生长情况。方法 小鼠胚胎干细胞 (ESD3细胞株 )在以下六种培养体系中培养 :1 .原代小鼠胚胎成纤维细胞 (MEF)有血清培养 ,2 .MEF无血清培养 ,3.SNL细胞有血清培养 ,4.LIF(白血病抑制因子 )...
关键词:观察 干细胞 血清培养 ES细胞 裸小鼠 饲养层 集落 克隆形态 小鼠胚胎 培养体系 
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