国家自然科学基金(10979005)

作品数：6被引量：16H指数：1
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相关作者：李艳华孔敏敏王文雅董宇辉袁其朋更多>>
相关机构：北京化工大学中国科学院更多>>
相关期刊：《中医学报》《Chinese Physics C》更多>>
相关主题：SAXSSMALL-ANGLEPROGRAMDRDIRECT更多>>
相关领域：理学机械工程自动化与计算机技术生物学更多>>
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Small-angle X-ray scattering probe of intermolecular interaction in red blood cells
《Chinese Physics C》2015年第3期83-86,共4页刘广峰 王文佳 徐建华 董宇辉 
Supported by National Basic Research Program of China(2009CB918600);National Natural Science Foundation of China(10979005)
With high concentrations of hemoglobin （Hb） in red blood cells, self-interactions among these molecules could increase the propensities of their polymerization and aggregation. In the present work, high concentratio...
关键词：small-angle X-ray scattering protein-protein interaction HEMOGLOBIN 
A program for SAXS data processing and analysis被引量：14
《Chinese Physics C》2013年第10期110-115,共6页李志宏 
National Basic Research Program of China(2011CB911104);National Natural Science Foundation of China (11079041,10835008,21176255,10979005,51002165,21276278,111107903928)
A computer program for small angle X-ray scattering （SAXS） data processing and analysis named S.exe written in Intel Visual Fortran has been developed. This paper briefly introduces its main theory and function.
关键词：SAXS data processing PROGRAM 
Solution of the effects of twinning in femtosecond X-ray protein nanocrystallography
《Chinese Physics C》2013年第2期105-110,共6页周亮 刘鹏 董宇辉 
Supported by National Natural Science Foundation of China (10979005);National Basic Research Program of China(2009CB918600)
With the development of the XFEL （X-ray free electron laser）, high quality diffraction patterns from nanocrystals have been achieved. The nanocrystals with different sizes and random orientations are injected to the...
关键词：coherent X-ray diffractive imaging XFEL oversampling technique protein nanocrystallography multiple-common-lines 
Direct demodulation method for heavy atom position determination in protein crystallography被引量：1
《Chinese Physics C》2013年第1期133-137,共5页周亮 刘忠川 刘鹏 董宇辉 
Supported by National Natural Science Foundation of China(10979005);National Basic Research Program of China(2009CB918600)
The first step of phasing in any de novo protein structure determination using isomorphous replacement （IR） or anomalous scattering （AD） experiments is to find heavy atom positions. Traditionally, heavy atom posit...
关键词：direct demodulation method heavy atom sites difference Patterson map protein crystallography 
Solution conformation of the response regulator proteins from Deinococcus radiodurans studied by SAXS被引量：1
《Chinese Physics C》2011年第10期974-977,共4页李丽琴 刘颖 刘鹏 董宇辉 
Supported by National Natural Science Foundation of China(10979005);National Basic Research Program of China(2009CB918600)
In this paper the solution conformation of the response regulator proteins from Deinococcus radiodurans was studied by small-angle X-ray scattering （SAXS）. The SAXS curves of Dr-rrA in solutions were obtained at Bea...
关键词：small-angle X-ray scattering response regulator Dr-rrA solution conformation 
大肠杆菌DNA复制相关蛋白PriC的初步研究
《中医学报》2011年第6期689-692,共4页孔敏敏 李艳华 董宇辉 王文雅 袁其朋 
国家自然科学基金(编号:10979005);国家基础研究项目(编号:2009CB918600)
目的:对大肠杆菌DNA复制相关蛋白PriC进行研究。方法:构建重组表达载体pET21a-PriC及pET21a-PriC1-101,并进一步诱导表达纯化。利用Pulldown手段将pET21a-PriC-notag与pET28at-plus-PriB分别转化到大肠杆菌BL21(DE3)中,诱导表达及共纯...
关键词：大肠杆菌 DNA 蛋白PriC 
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