supported by the National Key Research and Development Program(2019YFA0110800 and 2020YFA0707900 to W.L.and 2018YFA0108400 and 2019YFA0903800 to Q.Z.);the Strategic Priority Research Programof the Chinese Academy of Sciences(XDA16030403 to W.L.);the National Natural Science Foundation of China(31621004 to Q.Z.and W.L.);and the CAS Project for Young Scientists in Basic Research(YSBR-012 to W.L.).
The naturally occurring prokaryotic CRISPR-Cas systems provide valuable resources for the development of new genome-editing tools.However,the majority of prokaryotic Cas nucleases exhibit poor editing efficiency in ma...