This work was supported by a Longer and Larger(LoLa)grant from the Biotechnology and Biological Sciences Research Council(BBSRC,grant numbers BB/G020744/1,BB/G019177/1,BB/G019274/1 and BB/G018553/1);the UK Department for Environment,Food and Rural Affairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.Funding for LZ was provided by the BBSRC(grant number BB/C508193/1);The funders had no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.
Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as T...