A large number of Chinese herbal drugs (CHDs) exhibit antibacterial activities both in vivo and in vitro, but until now little is known regarding their inhibitory mechanisms. Bacterial DNA gyrase is a proven target fo...
supported by the National Natural Science Foundation of China (10834014,10974248);the National Basic Research Program of China (2009CB930704)
The DNA gyrase of Escherichia coli plays an essential role in the life of this microorganism.It is unique among all topoisomerases because of its ability to introduce negative supercoils into DNA.This study investigat...
We developed a novel PCR method aimed at identi- fying and amplifying native codon sequences of muta- tion-prone amino acids in DNA gyrase implicated in quinolone resistance using a naturally occurring co- don bias in...
This work was supported by the grants from the National Natural Science Foundation of China (No.30170512);the Major State Basic Research Development Program of China (No.2001cb108901)
In prokaryotes,DNA supercoiling regulates the expression of many genes;for example,the expression of Klebsiella pneumoniae nifLA operon depends on DNA negative supercoiling in anaerobically grown cells,which indicates...