MONOCOT

作品数：21被引量：436H指数：6
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相关期刊：《Science China(Life Sciences)》《Plant Diversity》《American Journal of Plant Sciences》《Wuhan University Journal of Natural Sciences》更多>>
相关基金：国家自然科学基金国家重点基础研究发展计划江苏省自然科学基金更多>>
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Optimized protoplast isolation and transfection with a breakpoint:accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot
《aBIOTECH》2024年第2期151-168,共18页Debasmita Panda Subhasis Karmakar Manaswini Dash Swagat Kumar Tripathy Priya Das Sagar Banerjee Yiping Qi Sanghamitra Samantaray Pradipta Kumar Mohapatra Mirza J.Baig Kutubuddin A.Molla 
support from the University Grant Commission(UGC);funding support from the Science and Engineering Research Board(SERB)-National Post-Doctoral Fellowship.;funding support from the Department of Biotechnology(DBT),Government of India-JRF program.MD,SB,MJB;funding from the Indian Council of Agricultural Research(ICAR),New Delhi,in the form of the Plan Scheme-‘Incentivizing Research in Agriculture’project;support from the Director,NRRI.
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic loci.Software-predicted guide RNAs(gRNAs)often fail to induce ...
关键词：PROTOPLAST Genome editing Transgene-free edited plants Rice CHICKPEA 
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