目的构建PIAS-NY(protein inhibitor of activated STAT-NY,PIAS-NY)的诱饵载体,为应用酵母双杂交系统筛选与PIAS-NY相互作用的蛋白建立实验基础。方法应用PCR法扩增PIAS-NY编码序列的片段,先将其克隆入pGEMT-easy内,经序列测定确认无误...
Objective To investigate the function and possible mechanisms of PIAS3 expression on the invasion of TJ905 cells. Methods PIAS3 overexpression vectors were constructed and PIAS3 siRNA were chemically synthesized, whic...