PICHIA_PASTORIS

作品数：195被引量：497H指数：10
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Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris
《Synthetic and Systems Biotechnology》2024年第4期759-765,共7页Shupeng Ruan Yuxin Yang Xinying Zhang Guanjuan Luo Ying Lin Shuli Liang 
supported by the National Key Research and Development Program(2021YFC2104000);the National Natural Science Foundation of China(32272276);the Fundamental Research Funds for the Central Universities.
Pichia pastoris,a methylotrophic yeast,can utilize methanol as a carbon source and energy source to synthesize high-value chemicals,and is an ideal host for biomanufacturing.Constructing the P.pastoris cell factory is...
关键词：P.PASTORIS CRISPR-Cpf1 Integration sites Metabolic engineering 
Combined strategies for improving the heterologous expression of a novel xylanase from Fusarium oxysporum Fo47 in Pichia pastoris
《Synthetic and Systems Biotechnology》2024年第3期426-435,共10页Chun Liu Yaping Zhang Chunting Ye Fengguang Zhao Yian Chen Shuangyan Han 
supported by the Key-Area Research and Development Program of Guangdong Province(2020B020226007);National Key Research and Development Program of China(2021YFC2100405,2022YFC2105501).
Xylanase,an enzyme capable of hydrolyzing non-starch polysaccharides found in grain structures like wheat,has been found to improve the organizational structure of dough and thus increase its volume.In our past work,o...
关键词：XYLANASE Pichia pastoris Heterologous protein Secretion expression 
Pichia pastoris composition expressed aerolysin mutant of Aeromonas veronii as an oral vaccine evaluated in zebrafish(Danio rerio)
《Marine Life Science & Technology》2024年第3期475-487,共13页Yuan-Yuan Yao Qing-Shuang Zhang Shu-Bin Liu Hong-Wei Yang Xing-Yu Chen Ya-Lin Yang Chen-Chen Gao Chao Ran Tsegay Teame Zhen Zhang Zhi-Gang Zhou 
funded by National Natural Sci-ence Foundation of China(NSFC 32303027);Agricultural Science and Technology Innovation Program(ASTIP)and Basal Research Fund(1610382023017)of the Chinese Academy of Agricultural Sciences;Project Team of Foshan National Hi-tech Industrial Development Zone Industrialization Entrepreneurial Teams Program(2320197000012);Beijing Innovation Consortium of Agriculture Research System(BAIC07-2023-11).
Vaccines are one of the most practical means to stop the spreading of Aeromonas veronii in aquaculture.In this study,virulence factor aerolysin mutant NTaer which has lost its hemolytic activity was used as a target a...
关键词：Aeromonas veronii Aerolysin mutant NTaer Oral vaccine Immune response Gut microbiota 
The Pichia pastoris enzyme production platform:From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass
《Journal of Bioresources and Bioproducts》2024年第1期43-57,共15页Korbinian Sinzinger Ulrike Obst Samed Güner Manuel Döring Magdalena Haslbeck Doris Schieder Volker Sieber 
The demand for industrial enzymes is continually rising,fueled by the growing need to shift towards more sustainable industrial processes.However,making efficient enzyme production strains and identifying optimal enzy...
关键词：Protein secretion PHYTASE Cyanobacterial biomass Fed-batch fermentation Pichia pastoris 
Expression and antimicrobial activity of the recombinant bovine lactoferricin in Pichia pastoris
《Synthetic and Systems Biotechnology》2024年第1期26-32,共7页Xueqin Lv Yuting Zhang Lingrui Wang Shixiu Cui Yanfeng Liu Jianghua Li Guocheng Du Long Liu 
supported by the National Natural Science Foundation of China(32021005,32270096);the Fundamental Research Funds for the Central Universities(JUSRP222007,JUSRP622004).
Lactoferricin,a multifunctional peptide located in the N-terminal region of lactoferrin,has a broad-spectrum bacteriostatic activity.It is a promising candidate as a food additive and immune fortification agent and do...
关键词：Antimicrobial peptide LACTOFERRICIN Recombinant expression Pichia pastoris 
Efficient expression of an alkaline pectin lyase from Bacillus licheniformis in Pichia pastoris
《Bioresources and Bioprocessing》2024年第1期526-540,共15页Junyi Li Manli Yang Fengguang Zhao Yaping Zhang Shuangyan Han 
supported by the National Key R&D Program of China(NO.2021YFC2100400).
Pectin lyase(PMGL)is an industrially important enzyme with widespread applications in the food,paper,and textile industries,owing to its capacity for direct degradation of highly esterified pectin.In this study,PMGL-B...
关键词：Alkaline pectin lyase Efficient expression Pichia pastoris 
Expression,characterization,and application of human-like recombinant gelatin
《Bioresources and Bioprocessing》2024年第1期933-945,共13页Xiaoping Song Tao Chu Wanru Shi Jingyan He 
financially supported by the Anhui Provincial Natural Science Foundation(No.2022AH052316,GXXT-2022-002,KJ2021A1273).
Gelatin is a product obtained through partial hydrolysis and thermal denaturation of collagen,belonging to natural biopeptides.With irreplaceable biological functions in the field of biomedical science and tissue engi...
关键词：Recombinant human gelatin Monomer protein sequence Pichia pastoris CHARACTERIZATION Three-dimensional culture Biomedical materials 
Genetic tools for metabolic engineering of Pichia pastoris被引量：2
《Engineering Microbiology》2023年第4期59-66,共8页Xiaoyan Wu Peng Cai Lun Yao Yongjin J Zhou 
supported by supported by the National Key R&D Program of China(2021YFC2103500);DICP innovation grant(DICP I202111)from Dalian Institute of Chemical Physics,CAS.
The methylotrophic yeast Pichia pastoris(also known as Komagataella phaffii)is widely used as a yeast cell factory for producing heterologous proteins.Recently,it has gained attention for its potential in producing ch...
关键词：Pichia pastoris Genetic tools Metabolic engineering CRISPR/Cas9 
Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs被引量：5
《Synthetic and Systems Biotechnology》2023年第3期396-415,共20页Arslan Sarwar Eun Yeol Lee 
the C1 Gas Refinery Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Science and ICT(2015M3D3A1A01064882);the Korea Institute of Marine Science&Technology Promotion(KIMST),funded by the Ministry of Oceans and Fisheries,Korea(20220532).
Methanol has recently gained significant attention as a potential carbon substrate for the production of fuels and chemicals,owing to its high degree of reduction,abundance,and low price.Native methylotrophic yeasts a...
关键词：Native methylotrophs Synthetic methylotrophs Pichia pastoris Methylobacterium extorquens Bacillus methanolicus Escherichia coli 
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris被引量：2
《Synthetic and Systems Biotechnology》2023年第3期445-451,共7页Xiang Wang Yi Li Zhehao Jin Xiangjian Liu Xiang Gao Shuyuan Guo Tao Yu 
Key-Area Research and Development Program of Guangdong Province(2022B1111080005);the National Key Research and Development Program of China(2020YFA0907800 and 2021YFA0911000);the National Natural Science Foundation of China(NSFC 32071416);the Shenzhen Institute of Synthetic Biology Scientific Research Program(Grant No.JCHZ20200003);Shenzhen Key Laboratory for the Intelligent Microbial Manufacturing of Medicines(ZDSYS20210623091810032);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0480000);the China Postdoctoral Science Foundation(2020M682973);Guangdong Basic and Applied Basic Research Foundation(2020A1515110927).
The methylotrophic budding yeast Pichia pastoris has been utilized to the production of a variety of heterologous recombinant proteins owing to the strong inducible alcohol oxidase promoter(pAOX1).However,it is diffic...
关键词：Pichia pastoris Uracil auxotroph CRISPR/Cas9 Recyclable selective marker Homology directed repair 
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