the National Natural Science Foundation of China(U1603234);the 948 Project from the Ministry of Agriculture of China(2012-S12);the Project for the Key Science and Technology Innovation Team of Shaanxi Province,China(2013KCT-25);the Key Research and Development Plan of Ningxia Hui Autonomous Region,China(2019BEF02005)。
Grapevine(Vitis spp.)is one of the most economically important fruit crops worldwide,and there is considerable interest in improving its major agronomic and enological traits in response to ever-changing agricultural ...
financially supported by the National Natural Science Foundation of China(31401438);the Innovation Research Team of the Ministry of Education of China(IRT_17R45);the earmarked fund for China Agriculture Research System(CARS-11-GXLJ);the Guangxi Scientific and Technological Development Subject,China(AB16380080 and AB16380163)
Protoplast electrofusion between callus protoplasts of cultivar TMS60444 and mesophyll protoplasts of cultivar SC8 was performed as an approach for the genetic improvement of cassava.The fusion products were subsequen...
financially supported by grants from the Biogreen 21 Program, RDA, Korea (PJ00810304);the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences (2014–2015);the Beijing Municipal Education Commission, China (KM200910011001)
Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major...
supported by a grant from the Bill Melinda Gates Foundation;National Institute of Food and Agriculture of the United States Department of Agriculture for support (Award number 2013-33522-21091)
Switchgrass is native to the tallgrass prairie of North America. It is self-incompatible and has varied ploidy levels from diploid(2x) to dodecaploid(12x) with tetraploid and octoploid being the most common. The h...
financially supported in part by the National Key Project for Tansgenic Study, Ministry of Agriculture of China(2011ZX08010-004)
The regeneration rate of wheat immature embryo varies among genotypes, howbeit many elite agriculture wheat varieties have low regeneration rates. Optimization of tissue culture conditions and attempts of adding signa...
the National Natural Science Foundation of China(30971776);the Transgenic Major Projects,Ministry of Agriculture of China(2011ZX08010-004)
The immature embryos (IEs) of wheat are the most widely used tissues for in vitro culture and genetic transformation due to its high regeneration competency. However, this explant can only be maintained in 4℃ daily...
To determine the most effective dose of arabinogalactan-protein (AGP) in regeneration medium, mature embryos of genotypes in three different ploidy levels (Triticum aestivum L. cv. Ikizce-96, Triticum durum Desf. c...
supported by the Key Project for International Cooperation and Exchanges,National Natural Science Foundation of China (30810103911);the National Natural Science Foundation of China(30771368);the Doctoral Fund of Ministry of Education for Young Scholar,China (20070504087)
The green-fluorescent protein (gfp) gene was evaluated as a screening marker during cotton (Gossypium hirsutum L.) transforming and plant regeneration. High expression of GFP (green-fluorescent protein) was obse...