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Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs被引量：1
《Journal of Animal Science and Biotechnology》2024年第4期1550-1558,共9页Marc Llavanera Yentel Mateo‑Otero Estel Vinolas‑Vergés Sergi Bonet Marc Yeste 
funded by the Ministry of Science and Innovation,Spain (AGL2017-88329-R, FPU18/00666 and PID2020-113320RB-I00);the Regional Government of Catalonia,Spain (2017-SGR-1229, 2020-FI-B-00412 and 2020-SGR-0900);the Catalan Institution for Research and Advanced Studies (ICREA)。
Background Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, includ-ing energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content(mtDNA...
关键词：FERTILITY MITOCHONDRIA MOTILITY mtDNA copy number qPCR SPERM 
Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction
《Journal of Animal Science and Biotechnology》2024年第1期171-181,共11页Estel Viñolas-Vergés Jordi Ribas-Maynou Isabel Barranco Camila Peres Rubio Sergi Bonet Jordi Roca Marc Yeste 
This research was supported by the European Union’s Horizon 2020 research and innovation scheme under the Marie Skłodowska-Curie grant agreement No.801342(Tecniospring INDUSTRY;Grant:TECSPR-19-1-0003);the Ministry of Science and Innovation,Spain(Grants:PID2020-113320RB-I00,PID2020-113493RB-I00,RYC2021-034546-I and RYC2021-034764-I);the Catalan Agency for Management of University and Research Grants,Regional Government of Catalonia,Spain(Grants:2017-SGR-1229 and 2021-SGR-00900);the Seneca Foundation,Regional Government of Murcia,Spain(Grant:21935/PI/22);La Marato de TV3 Foundation(Grant:214/857-202039);and the Catalan Institution for Research and Advanced Studies(ICREA).
Background Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo development.In some mammals,like pigs,ejaculates are emitted in three separa...
关键词：CHROMATIN Condensation DNA integrity Ejaculate fractions PIG Protamination SPERM 
Expression of miR‑138 in cryopreserved bovine sperm is related to their fertility potential
《Journal of Animal Science and Biotechnology》2023年第6期2383-2394,共12页Albert Salas‑Huetos Jordi Ribas‑Maynou Yentel Mateo‑Otero Carolina Tamargo Marc Llavanera Marc Yeste 
the Ministry of Science and Innovation,Spain(IJC2019-039615-I and PID2020-113320RB-I00);the European Union’s Horizon 2020 research and innovation scheme under the Marie Sklodowska-Curie grant agreement No.801342(Techniospring INDUSTRY;TECSPR-19-1-0003);the Regional Government of Catalonia(2017-SGR-1229 and 2021-SGR-00900);the Catalan Institution for Research and Advanced Studies(ICREA).
Background MicroRNAs(miRNAs)are small,single-stranded,non-coding RNA molecules of 22–24 nucleotides that regulate gene expression.In the last decade,miRNAs have been described in sperm of several mammals,including ca...
关键词：BOVINE FERTILITY MIRNAS SPERM 
Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams
《Journal of Animal Science and Biotechnology》2023年第6期2395-2410,共16页Alejandro Jurado‑Campos Pedro Javier Soria‑Meneses Maria Arenas‑Moreira Carlos Alonso‑Moreno Virginia Rodriguez‑Robledo Ana Josefa Soler Jose Julian Garde Maria del Rocio Fernandez‑Santos 
the financial support,grants AGL2017-85603-P,PID2020-120281RB-100 and PID2020-117788RB-100 funded by MCIN/AEI/10,13039/501100011033;grants SBPLY/21/180501/000111 and SBPLY/21/180501/000050 funded by JCCM by EU through Fondo Europeo de Desarrollo Regional;supported by a UCLM scholarship;supported by a JCCM scholarship
Background Artificial insemination(AI)is a routine breeding technology in animal reproduction.Nevertheless,the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI.In this ...
关键词：Breeding technology NANOEMULSIONS NANOTECHNOLOGY Sperm oxidative stress Vitamin E 
Determination of double-and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity
《Journal of Animal Science and Biotechnology》2023年第1期134-151,共18页Jordi Ribas‑Maynou Ariadna Delgado‑Bermúdez Yentel Mateo‑Otero Estel Vinolas Carlos O.Hidalgo WSteven Ward Marc Yeste 
the European Union’s Horizon 2020 Research and Innovation scheme under the Marie Sklodowska-Curie grant agreement No.801342(Tecniospring INDUSTRY,TECSPR-19-1-0003);the Ministry of Science and Innovation,Spain(AGL2017-88329-R and PID2020-113320RBI00);the Catalan Agency for Management of University and Research Grants,Regional Government of Catalonia,Spain(2017-SGR-1229);the Catalan Institution for Research and Advanced Studies(ICREA);La Maratóde TV3 Foundation(214/857-202039)。
Background:The analysis of chromatin integrity has become an important determinant of sperm quality.In frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of s...
关键词：Cattle CHROMATIN Comet test DNA damage FERTILITY SPERM Sperm quality 
Establishment of a male fertility prediction model with sperm RNA markers in pigs as a translational animal model
《Journal of Animal Science and Biotechnology》2022年第6期1743-1753,共11页Won-Ki Pang Shehreen Amjad Do-Yeal Ryu Elikanah Olusayo Adegoke Md Saidur Rahman Yoo-Jin Park Myung-Geol Pang 
supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Education(NRF-2018R1A6A1A03025159).
Background:Male infertility is an important issue that causes low production in the animal industry.To solve the male fertility crisis in the animal industry,the prediction of sperm quality is the most important step....
关键词：Male fertility Multiple markers Pig model Prediction model Sperm RNA 
Heat shock protein family D member 1 in boar spermatozoa is strongly related to the litter size of inseminated sows被引量：1
《Journal of Animal Science and Biotechnology》2022年第4期1045-1055,共11页Won-Ki Pang Ji-Hyun Son Do-Yeal Ryu Md Saidur Rahman Yoo-Jin Park Myung-Geol Pang 
supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Education(NRF-2018R1A6A1A03025159).
Background:Sperm quality evaluation is the logical first step in increasing field fertility.Spermatozoa contain cytoplasmic organelles and biomolecules known as sperm-intrinsic factors,which play key roles in sperm ma...
关键词：FERTILISATION HSPD1 Male fertility Sperm motility Sperm RNA 
TLR7/8 signalling affects X-sperm motility via the GSK3α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos被引量：5
《Journal of Animal Science and Biotechnology》2022年第2期401-417,共17页Fa Ren Huaming Xi Yijie Ren Yu Li Fei Wen Ming Xian Mengjie Zhao Dawei Zhu Liqiang Wang Anmin Lei Jianhong Hu 
This research was supported by the National Natural Science Foundation of China(31672425);Shaanxi Province Key R&D Program(2018ZDXM-NY-043,2020ZDLNY02–04).
Background:Goat milk is very similar to human milk in terms of its abundant nutrients and ease of digestion.To derive greater economic benefit,farmers require more female offspring(does);however,the buck-to-doe offspr...
关键词：Dairy goat Glycogen synthase kinaseα/β(GSK3α/β) Sexing control SPERM Toll-like receptor 7/8(TLR7/8) 
Expression,structure and function analysis of the sperm-oocyte fusion genes Juno and Izumo1 in sheep(Ovis aries)被引量：3
《Journal of Animal Science and Biotechnology》2021年第3期917-934,共18页Wenping Hu Xinlong Dong Zhilong Tian Zhuangbiao Zhang Jishun Tang Benmeng Liang Qiuyue Liu Mingxing Chu 
This research was funded by National Natural Science Foundation of China,grant number 31501941;Central Public-interest Scientific Institution Basal Research Fund,grant number 2018-YWF-YB-1,and 2015ywf-zd-2;the Earmarked Fund for China Agriculture Research System,grant number CARS-38;the Agricultural Science and Technology Innovation Program of China,grant number ASTIP-IAS13.
Background:JUNO and IZUMO1 are the first receptor-ligand protein pairs discovered to be essential for spermoocyte fusion;their interaction is indispensable for fertilization.Methods:PCR was used to clone the full-leng...
关键词：FERTILIZATION Izumo1 Juno SHEEP Single cell RNA-seq 
GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm被引量：4
《Journal of Animal Science and Biotechnology》2019年第4期931-941,共11页Marc Llavanera Ariadna Delgado-Bermúdez Beatriz Fernandez-Fuertes Sandra Recuero Yentel Mateo Sergi Bonet Isabel Barranco Marc Yeste 
support from Ministry of Science,Innovation and Universities,Spain(Grants:RYC-2014-15581,AGL2017–88329-R and FJCI-2017-31689);European Commission(H2020-MSCA-IF-79212)
Background: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination(AI) centres. However, it is well-known that ...
关键词：BOAR CRYOPRESERVATION GSTM3 IZUMO1 ROS SPERM 
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