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CLONING: 作品数：956被引量：1822H指数：15; 导出分析报告; 相关领域：生物学医药卫生更多>>; 相关作者：庹德财沈文涛黎小瑛周鹏言普更多>>; 相关机构：中国科学院遗传与发育生物学研究所中国热带农业科学院吉林大学中国科学院更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划广东省自然科学基金更多>>

Cloning,expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug,Adelphocoris lineolatus(Goeze)被引量：8: 《Chinese Science Bulletin》2010年第34期3911-3921,共11页GU ShaoHua SUN Yang REN LiYan ZHANG XueYing ZHANG YongJun WU KongMing GUO YuYuan; supported by the National Key Basic Research Program of China(2007CB109202);the National High-Technology Research and Development Program of China(2008BAD5B05);National Natural Science Foundation of China(31071694); Credible evidence shows that odorant binding proteins(OBPs)are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors(OR...; 关键词：气味结合蛋白克隆和表达结合特异性线纹结合亲和力植物挥发物性信息素解离常数

Cloning of 9-cis-epoxycarotenoid dioxygenase (NCED) gene encoding a key enzyme during abscisic acid (ABA) biosynthesis and ABA-regulated ethylene production in detached young persimmon calyx被引量：16: 《Chinese Science Bulletin》2009年第16期2830-2838,共9页LENG Ping ZHANG GuangLian LI XiangXin WANG LiangHe ZHENG ZhongMing; Supported by the Beijing Natural Science Foundation (Grant No. 6052013);Beijing Municipal Science and Technology Commission (Grant No. D0706002000091); Unlike the typical climacteric fruits, persimmons (Diospyros kaki Thunb.) produce higher levels of ethylene when they are detached from trees at a younger stage. In order to obtain detailed information on the role of ...; 关键词：ABA调节基因编码双加氧酶关键酶脱落酸生产乙烯合成

Evolutionary analysis of allotetraploid hybrids of red crucian carp×common carp,based on ISSR,AFLP molecular markers and cloning of cyclins genes被引量：6: 《Chinese Science Bulletin》2009年第16期2849-2861,共13页LIU LiangGuo YAN JinPeng LIU ShaoJun LIU Dong YOU CuiPing ZHONG Huan TAO Min LIU Yun; Supported by the National Natural Science Fund for Distinguished Young Scholars (Grant No. 30725028);State Key Basic Research and Development Program of China (Grant No. 2007CB109206);Specially-appointed Professor for Lotus Scholars Program of Hunan Province; The allotetraploid hybrids of red crucian carp × common carp are the first reported artificially cultured polyploid fish with bisexual fertility and stable inheritance in vertebrate. Using ISSR and AFLP markers and t...; 关键词：异源四倍体鲫鲤细胞周期蛋白基因克隆 AFLP分子标记 ISSR 杂交种 DNA指纹图谱分析进化

Cloning of genes encoding nonhost hypersensitive response-inducing elicitors from Phytophthora boehmeriae: 《Chinese Science Bulletin》2007年第2期231-237,共7页LI Jun ZHANG HaiFeng ZHANG ZhengGuang WANG YuanChao ZHENG XiaoBo; the Nation Natural Science Foundation of China (Grant No. 30300228);the National Research Foundation for the Doctoral Program of Higher Education of China (Grant No. 20020307035);the Student Research Training of Nanjing Agricultural University (Grant No. 0402A04); We have devised a high-throughput functional cloning method to isolate cDNAs from Phytophthora boehmeriae of which the products elicit a hypersensitive response (HR) in tobacco. The cDNAs were cloned into a binary pot...; 关键词：基因工程克隆技术敏感机制烟草基因编码

Cloning of the key genes in maize oxylipins pathways and their roles in herbivore induced defense被引量：2: 《Chinese Science Bulletin》2005年第21期2457-2466,共10页XU Tao WANG Jianwu LUO Shiming; supported by the National Natural Science Foundation of China(Grant No.30000114,30270137);Natural Science Foundation of Guangdong Province(Grant No.039254,04020569&21043); To gain an understanding of the molecular ba-sis of signaling pathways in herbivore-induced maize plant defense, three key genes, ZmAOS, ZmAOC and ZmHPL, which are involved in the biosynthesis of oxylipin signals, hav...; 关键词：玉米繁殖技术基因表达茉莉酮

Cloning and characterization of a gene encoding cysteine proteases from senescent leaves of Gossypium hirsutum被引量：7: 《Chinese Science Bulletin》2004年第24期2601-2607,共7页SHENFafu YUShuxun HANXiulan FANShuli; A gene encoding a cysteine proteinase was iso- lated from senescent leave of cotton (Gossypium hirsutum) cv liaomian No. 9 by utilizing rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR), and a set ...; 关键词：编码巯基丙氨酸蛋白酶树叶基因无性繁殖系统遗传控制系统细胞植物

Cloning,sequencing and expression analysis of cDNA encoding a constitutive heat shock protein 70 (HSC70) in Fenneropenaeus chinensis被引量：8: 《Chinese Science Bulletin》2004年第22期2385-2393,共9页JIAOChuanzhen WANGZaizhao LIFuhua ZHANGChengsong XlANGJianhai; The cDNA encoding hsc70 of Chinese shrimp Fenneropenaeus chinensis was cloned from hepatopancreas by RT-PCR based on its EST sequence. The full length cDNA of 2090 bp contained an open reading frame of 1956 nucleotide...; 关键词：无性繁殖 cDNA 表达方式编码热震动蛋白质70 HSC70 虾

Cloning and analysis of expression profile of 13 WRKY genes in rice被引量：49: 《Chinese Science Bulletin》2004年第20期2159-2168,共10页QIUYuping JINGShaojuan FUJian LILu YUDiqiu; The transcriptional factor WRKY proteins contain the highly conserved amino acid sequence WRKYGQK as well as the novel zinc-finger-like motifs Cys2His2 or Cys2HisCys. A search of the rice genome identi-fied 97 genes e...; 关键词：无性繁殖稻基因表达 WRKY基因转录因子非生物应激因子转基因作物

Cloning and analysis of the antagonistic related genes of Enterobacter cloacae B8被引量：2: 《Chinese Science Bulletin》2004年第13期1370-1375,共6页YUXuping ZHUJunli YAOXunping HEShicheng HUANGHaining CHENWeiliang LIDebao; To understand the antagonistic mechanism of the broad spectrum antagonistic Enterobacter cloacae B8,Tn5 transposon-mediated mutagenesis is performed using suicide plasmid pZJ25. Two mutant strains that lost antagonist...; 关键词：稻无性繁殖拮抗机理 DNA 肠细菌学B8 生物控制生物合成酶药物残留

Cloning and roles of goldfish maternal factor β-Catenin cDNA in embryonic development: 《Chinese Science Bulletin》2004年第7期683-688,共6页ZHANG Jingpu1,2,WANG Weixian1 & ZHU Shaoxia1 1.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100080,China 2.The Graduate School of the Chinese Academy of Sciences,Beijing 100039,China Correspondence should be addressed to Zhang Jingpu; Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. b-Catenin, an important maternal factor in early embr...; 关键词：胚胎发育母体因子金鱼基因克隆氨基酸序列 CDNA 细胞质细胞核

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