GENE_CLONING

作品数：174被引量：328H指数：8
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Molecular Cloning and Bioinformatics Analysis of msrA Gene from Vibrio alginolyticus Strain HY9901
《Agricultural Biotechnology》2024年第4期1-5,10,共6页Zhiqing WEI Zhihang CHEN Yingzhu WEI Na WANG Huanying PANG 
Supported National Natural Science Foundation of China(32073015);Undergraduate Training Program for Innovation and Entrepreneurship of Guangdong Ocean University(CXXL2024007);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802);Postgraduate Education Innovation Program of Guangdong Ocean University(No.202446);Postgraduate Education Innovation Program of Guangdong Province(YJYH[2022]1).
[Objectives]This study was conducted to understand the structure and function of MsrA protein.[Methods]With Vibrio alginolyticus HY9901 as the object of study,primers were designed to amplify the full-length gene of m...
关键词：Vibrio alginolyticus Gene cloning MSRA Bioinformatics analysis 
Research Progresses on QTLs for Main Grain Shape Genes in Rice
《Agricultural Biotechnology》2021年第6期7-9,13,共4页Mengxi ZHOU Diandong WANG Zhen'an BAI Ying PENG 
The performance of rice quality affects the market competitiveness of rice to a certain extent,and grain shape is an important factor for yield traits and appearance quality.The mapping and cloning of major rice grain...
关键词：RICE Rice grain quality Grain size Quantitative trait locus(QTL) Gene cloning 
Analysis of Cloning and Expression Characteristics of Capsicum chinense Jacq. CcMYB Gene
《Agricultural Biotechnology》2018年第4期1-3,7,共4页Ziqi ZHAO Yucen GUO Wanying ZHANG Zihan LI Haoyun SUN Chunyu ZHANG Jingying WANG Qingxun GUO 
Supported by College Students'Innovation Fund of Jilin University(2017A82372)
In order to discuss the role of MYB gene in capsaicine synthesis process, one CcMYB gene was cloned from Capsicum chinense Jacq. by RT-PCR. Its cDNA has a total length of 1 038 bp, and was speculated to code 345 amino...
关键词：Capsicum chinense Jacq. MYB Gene cloning EXPRESSION 
Cloning and Expression Analysis of Allene Oxide Synthase Gene Ls AOS in Lilium‘Siberia'被引量：1
《Agricultural Biotechnology》2018年第2期5-9,31,共6页Qi WU Pingsheng LENG Zenghui HU 
Supported by Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges in Beijing Municipality(DHT20150503);National Natural Science Foundation of China(31201645;31640070)
[Objectives]Allene oxide synthase(AOS),the first specific enzyme in the biosynthetic pathway of jasmonate(JA),has an important role in regulating JA accumulation in plants.This study was conducted to amplify full-leng...
关键词：Lilium'Siberia' LsAOS Gene cloning Expression analysisHome 
Genetic Analysis and Improvement Strategies of Protein Content in Rice Seeds被引量：1
《Agricultural Biotechnology》2017年第4期10-14,共5页Bo PENG Yanfang SUN Dongyan KONG Ruihua PANG Xiaohua SONG Huilong LI Lin LIU Xian-wen LI Jintao LI Qiying ZHOU Bin DUAN Huihui LIU Yuhan ZUO Yanlin HAO Shizhi SONG Hongyu YUAN 
Supported by National Natural Science Foundation of China(U1604110,U1404319,31270727,31600992);Key Project of Science and Technology in Henan Province(152102110100,152102110036);Nanhu Scholars Program for Young Scholars of XYNU(2016054);Major Science and Technology Project in Henan Province(121100110200);Science and Technology Innovation Team in Henan Province(14IRTSTHN012);Key Research Projects of Henan Higher Education Institutions(15A180059);Students Innovation and Training Program of XYNU(201710477032);Institute for Conservation and Utilization of Agrobioresources in Dabie Mountains(2016020)
Enhancing the nutritional quality of food crops is a way to improve human nutrition and health. Grain protein content （ GPC ） is an important nutritional quality trait for rice and many other crops. Therefore, it wi...
关键词：RICE Grain protein content Improvement strategy QTL mapping Gene cloning 
Cloning and Characterization of a Galactomannan-degrading Enzyme Gene in Pichia pastoris
《Agricultural Biotechnology》2015年第5期69-72,76,共5页Yuyong WU Jiao LIU Guangyun LU Jiahui LIU Xiaoli LIU 
Supported by Yantai Municipal Science and Technology Development Plan(2013ZH097);Scientific and Technological Innovation Fund for Students in Binzhou Medical University(BY2013DKCX122)
[ Objective ] This study aimed to obtain the recombinant Pichia yeast strain which can efficiently degrade guar gum. The properties of the recombinant enzyme were studied preliminarily. [ Method ] A positive clone tha...
关键词：Galactomarman-degrading enzyme Pichia pastoris Gene cloning Secreted expression Enzyme activity Enzymatie properties 
Responses of Drought-Resistant Mutant vem1 to Stress and Cloning of VEM1 Gene in Arabidopsis
《Agricultural Biotechnology》2013年第5期13-16,共4页Junxin SHI Jiaojiao ZHANG Ziping CHEN Xiaohui DING Jie YANG Li JIANG 
Supported by Key Project of Natural Science from the Education Department of Anhui Province(KJ2011ZD08);Anhui Provincial Natural Science Foundation(1208085MC47);National Innovation Training Program for College Students of China(201210359061)
[ Objective] This study aimed to investigate the responses of drought-resistant mutant veml to stress and clone VEM1 gene in Arabidopsis. [ Method] A drought-resistant mutant veml was isolated from the Arabidops/s mut...
关键词：ARABIDOPSIS Drought-resistant mutant veml STRESS Gene cloning 
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