云南高校图书馆联盟文献共享服务平台- GENE

GENE_CLONING: 作品数：174被引量：328H指数：8; 导出分析报告; 相关作者：高晓蓉安利佳李秋莉苏乔刘大伟更多>>; 相关机构：大连理工大学扬州大学连云港市农业局南京农业大学更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划广东省自然科学基金更多>>

Cloning and Bioinformatics Analysis of cobQ Gene from Vibrio alginolyticus Strain HY9901: 《Asian Agricultural Research》2025年第4期19-23,共5页Chao HUANG Zhihang CHEN Weijie ZHANG Xiaoxin WEN Jiajie MA Peng ZHOU Yingying JIANG Huanying PANG 无; Natural Science Foundation of Guangdong Province(2025A1515011061);Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean University;Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).; [Objectives] To analyze the function of cobQ gene from Vibrio alginolyticus strain HY9901,and to provide a reference for exploring the possible mechanism of cobQ gene from V.alginolyticus.[Methods] A pair of primers w...; 关键词：Vibrio alginolyticus Gene cloning cobQ gene Bioinformatics analysis

Cloning and Bioinformatics Analysis of CsFK111 Gene from Cucumbers: 《Journal of Northeast Agricultural University(English Edition)》2024年第4期16-30,共15页Zhang Hetong Li Li Gao Mei Jia Jincui Xin Ming; Supported by the National Natural Science Foundation of China(32272724);the National Science Foundation of Heilongjiang Province,China(LH2019C033)。; At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed gene CsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software too...; 关键词：F-box gene dwarf cucumber gene cloning bioinformatics analysis

Defect in an immune regulator gene BrSRFR1 leads to premature leaf senescence in Chinese cabbage: 《Horticultural Plant Journal》2024年第6期1414-1423,共10页Yue Xin Gengxing Song Chong Tan Hui Feng; supported by the National Natural Science Foundation of China (Grant No.31972405);Graduate Student Innovation Cultivation Project of Shenyang Agricultural University (Grant No.2021YCXB16)。; Leaf senescence is the final stage of leaf development, where the nutrients and energy of senescent leaves are redistributed to developing tissues or organs for plant growth, reproduction, and defense. Outer leaves ar...; 关键词：Chinese cabbage Premature leaf senescence SRFR1 Gene cloning

Molecular Cloning and Bioinformatics Analysis of msrA Gene from Vibrio alginolyticus Strain HY9901: 《Agricultural Biotechnology》2024年第4期1-5,10,共6页Zhiqing WEI Zhihang CHEN Yingzhu WEI Na WANG Huanying PANG; Supported National Natural Science Foundation of China(32073015);Undergraduate Training Program for Innovation and Entrepreneurship of Guangdong Ocean University(CXXL2024007);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802);Postgraduate Education Innovation Program of Guangdong Ocean University(No.202446);Postgraduate Education Innovation Program of Guangdong Province(YJYH[2022]1).; [Objectives]This study was conducted to understand the structure and function of MsrA protein.[Methods]With Vibrio alginolyticus HY9901 as the object of study,primers were designed to amplify the full-length gene of m...; 关键词：Vibrio alginolyticus Gene cloning MSRA Bioinformatics analysis

Molecular Cloning of sodB Gene from Vibrio alginolyticus HY9901 and Its Bioinformatics Analysis: 《Asian Agricultural Research》2024年第7期42-47,共6页Shuai YANG Yingying JIANG Haiyun FENG Weijie ZHANG Na WANG Xiaonan LU Huanying PANG; Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean University;National Natural Science Foundation of China(32073015);Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).; Vibrio alginolyticus is a zoonotic bacterium.A pair of specific primers was designed using the sodB gene sequence of Vibrio alginolyticus HY9901 in order to amplify the full length of the gene by PCR.The results indic...; 关键词：Vibrio alginolyticus Gene cloning sodB gene Bioinformatics analysis

Gene Cloning and Bioinformatics Analysis of phoR Gene from Vibrio alginolyticus HY9901: 《Asian Agricultural Research》2024年第6期36-40,共5页Xiangyu LIU Peng ZHOU Haiyun FENG Weijie ZHANG Huanying PANG Na WANG Xiaonan LU; Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean University;National Natural Science Foundation of China(32073015);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802);Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007).; PhoR is a histidine kinase in a two-component regulatory system that regulates phosphorus metabolic pathways and undertakes the key mission of information transmission in pathogenic bacteria.The full-length phoR gene ...; 关键词：VIBRIO ALGINOLYTICUS phoR GENE GENE CLONING BIOINFORMATICS analysis

Gene cloning,protein expression,and enzymatic characterization of a double-stranded RNA degrading enzyme in Apolygus lucorum: 《Insect Science》2024年第1期119-133,共15页Jie-Yu Zhang Jing Zhao Keyan Zhu-Salzman Qin-Qin Ji Yi-Ping Jiang Liu-Bin Xiao De-Jin Xu Guang-Chun Xu Lin-Quan Ge Yong-An Tan; This study was supported by Jiangsu Agricultural Science and Technology Innovation Fund[CX(21)3088,CX(22)2038];Jiangsu Province Key R&D Program(Modern Agriculture)Project:Surface Project(BE2021303).; RNA interference(RNAi)is a powerful tool that post-transcriptionally silences target genes in eukaryotic cells.However,silencing efficacy varies greatly among different insect species.Recently,we met with little succe...; 关键词：Apolygus lucorum CLONING dsRNase enzymatic activity protein expression

Mapping and Functional Analysis of LE Gene in a Lethal Etiolated Rice Mutant at Seedling Stage: 《Rice science》2023年第6期567-576,共10页XIA Xiaodong ZHANG Xiaobo WANG Zhonghao CHENG Benyi Sun Huifeng XU Xia GONG Junyi YANG Shihua WU Jianli SHI Yongfeng XU Rugen; supported by the National Natural Science Foundation of China(Grant No.32072049);Open Foundation of State Key Laboratory of Rice Biology,China(Grant No.20210208).; An EMS(ethy methanesulfonate)-induced lethal etiolated(le)mutant obtained from the rice variety Zhongjian 100 was characterized by lethal etiolated phenotypes,with significantly reduced levels of chlorophyll a,chlorop...; 关键词：Oryza sativa lethal etiolated mutant gene cloning functional analysis reactive oxygen species

Enhancing maize's nitrogen-fixing potential through ZmSBT3, a gene suppressing mucilage secretion∞: 《Journal of Integrative Plant Biology》2023年第12期2645-2659,共15页Jingyang Gao Peijiang Feng Jingli Zhang Chaopei Dong Zhao Wang Mingxiang Chen Zhongliang Yu Bowen Zhao Xin Hou Huijuan Wang Zhaokun Wu Razia Sultana Jemim Haidong Yu Doudou Sun Pei Jing Jiafa Chen Weibin Song Xuecai Zhang Zijian Zhou Jianyu Wu; the special fund at Henan Agricultural University; Maize(Zea mays)requires substantial amounts of nitrogen,posing a challenge for its cultivation.Recent work discovered that some ancient Mexican maize landraces harbored diazotrophic bacteria in mucilage secreted by th...; 关键词：MAIZE aerial root mucilage nitrogen‐fixing ZmSBT3 GWAS gene cloning DIAZOTROPH

BnGLO4 gene cloning and bioinformatics analysis in Brassica napus L: 《Oil Crop Science》2023年第3期133-142,共10页Xi Li Xiaodan Wang Shijun Li Zechuan Peng Dongfang Zhao Bingqian Zhou Chunyun Guan Mei Guan; This work was financially supported by China Agriculture Research System of MOF and MARA(CARS-13);Hunan Agriculture Research System of DARA.; High oleic acid rapeseed oil offers superior nutritional and health benefits,but its mass production is limited due to poor resistance and slightly lower yield.Photosynthesis serves as the foundation of biological sur...; 关键词：High oleic acid TRANSCRIPTOME Glycolate oxidase GLO4

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