ING1(inhibitor of growth 1)是一个候选抑癌基因家族,p47ING1a、p33ING1b和p24ING1c是其三种剪接异构体.通过MTT法和流式细胞术研究ING1a、ING1b和ING1c对HeLa细胞增殖的影响,结果发现三者均可将HeLa细胞阻滞于G0/G1期并抑制细胞生长....
Acknowledgements This work was supported by the National Key Basic Research Program of China (Grant Nos. 2002CB 513110 and 2004CB520804); the Key Technologies Research and Development Program (Grant No. 2003 BA310A 16); the International Key Corporation Project of the Chinese Technology Ministry (Grant No. 2003DF000039); the National Natural Science Foundation of China (Grant No. 20135010); the Key Project of Natural Science Foundation of Hunan Province (Grant No. 0399Y1006).
The effects of drug treatment, gene transfection, and p53 RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based...