This work was supposed by the National Natural ScienceFoundation of China (Grant Nos.39880003 and 20175016).
The hydrophobic amino acid residues of a denatured protein molecule tend to react with the particles of the stationary phase of hydrophobic interaction chromatography (STHIC). These hydrophobic interactions prevent th...
Human interferon-γ(IFN-γ)cDNA was synthesized,and it makes the usage of favorable codons in E.coli.The authors got 9 different expression plasmids which contain the synthetic IFN-γ-cDNA and have different spaces be...
Work supported by China National Expert Committee for Biotechnology Development
An interferon gamma (IFN-γ) mutant gene harboring an additionamal epidermal growth factor (EGF) sequence at its 3’ terminal was constructed and highly expressed in E. coli by using pBV220 as a vector. The new fusion...