supported by grants from The Fundamental Research Funds for the Central Universities(2015ZCQ-LY-02);The National Natural Science Foundation of China(31070651)~~
Supported by the National Natural Science Fundation Item of China(30970578,31070651);"Excellent Talent Support Plan in NewCentury"of Ministry of Education(NECT-08-0731)~~
[Objective] The research aimed to construct the prokaryotic expression vector of VP5 protein of IBDV.The transmembrane region sequence of VP5 protein was knocked out.Moreover,the expression,separation and purification...