一起肠炎沙门氏菌感染的食源性疾病暴发事件的溯源分析及药敏检测  

Traceability analysis and drug susceptibility detection of a food-borne disease outbreak with Salmonella enteritidis

作　　者：丁延辉 李成 王圣涵 许国峰 DING Yan-Hui;LI Cheng;WANG Sheng-Han;XU Guo-Feng(Zhifu Center for Disease Control and Prevention,Yantai 264001,China)

机构地区：[1]烟台市芝罘区疾病预防控制中心,烟台264001

出　　处：《实验室检测》2024年第2期98-101,共4页Laboratory Testing

摘　　要：目的对一起因食用肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件进行溯源分析。方法对8例患者进行流行病学调查,采集相关环境、食品及粪便等样本,根据国家标准进行实验室检测,经增菌后挑取可疑菌落进行生化鉴定,对检出的6株沙门氏菌株采用标准诊断血清进行血清凝集试验,药敏试验及脉冲场凝胶电泳。结果该起事件发病8例,6份样本中,分离出肠炎沙门氏菌,沙门氏菌检出率食品、环境和病人粪便样本均为100%,药敏试验结果显示,耐药谱为多粘菌素E(Colistin,CT)―头孢噻肟(Cefotaxime,CTX)―四环素(Tetracycline,TET)―环丙沙星(Ciprofloxacin,CIP)―氨苄西林(Ampicillin,AMP)―氨苄西林/舒巴坦(Ampicillin/Sulbactam,AMS),均呈现6重耐药现象,对氯霉素(Chloramphenicol,CHL)、复方新诺明(Sulfamethoxazole,SXT)、厄他培南(Ertapenem,ETP)、美罗培南(Meropenem,MEM)、头孢他啶(Ceftazidime,CAZ)、头孢他啶/阿维巴坦(Ceftazidime/avibactam,CZA)、替加环素(Tigecycline,TIG)、阿奇霉素(Azithromycin,AZM)、阿米卡星(Amikacin,AMI)敏感,6株菌的脉冲场凝胶电泳(Pulse Field Gel Electrophoresis,PFGE)条带一致,同源性100%,显示为同一克隆。结论该起事件为市民食用被肠炎沙门氏菌污染的熏肉大饼引起的食源性疾病暴发事件。Objective To trace the source of a foodborne disease outbreak caused by consuming smoked meat pancakes contaminated with Salmonella enteritidis.Methods Epidemiological investigations were carried out on 8 patients,samples of relevant environment,food and feces were collected,laboratory tests were carried out according to national standards.After bacteria enhancement,suspicious colonies were selected for biochemical identification,and the six strains of Salmonella detected underwent serum agglutination with standard diagnostic sera,drug sensitivity test and pulsed field gel electrophoresis.Result There were 8 cases of this incident,and out of the 6 samples,6 strains of Salmonella enteritidis were isolated.The detection rates of Salmonella in the food,environmental samples,and cases feces were all 100%.The drug sensitivity test results showed that all strains were resistant to 6 drugs,and the drug resistance spectrum was CT―CTX―TET―CIP―AMP―AMS.They were sensitive to CHL,SXT,ETP,MEM,CAZ,CZA,TIG,AZM,and AMI.The PFGE bands of the 6 strains were consistent,with the homology of 100%,and that showed the same clone.Conclusions This incident is a foodborne disease outbreak caused by consuming smoked meat pancakes contaminated with Salmonella enteritidis.

关 键 词：食源性疾病 肠炎沙门氏菌 溯源分析 药敏试验 脉冲场凝胶电泳 

分 类 号：R155.3[医药卫生—营养与食品卫生学]