Inhibitory Effects of Cypermethrin on Interactions of the Androgen Receptor with Coactivators ARA70 and ARA55  

作　　者：DING Zhen SHEN Jun Yu HONG Jia Wei ZHANG Rui LI Zheng WANG Qi ZHANG Jin Peng ZHANG Mei Rong XU Li Chun 

机构地区：[1]School of Public Health,Xuzhou Medical University,Xuzhou 221000,Jiangsu,China

出　　处：《Biomedical and Environmental Sciences》2020年第3期158-164,共7页生物医学与环境科学（英文版）

基　　金：supported by National Natural Science Foundations of China[No.81872647];the Natural Science Foundation of the Jiangsu Higher Education Institutions of China[19KJB330007].

摘　　要：Objective The purpose of this study was to investigate the anti-androgenic mechanism of cypermethrin involving coactivators.Methods Mammalian two-hybrid assays were performed to study the effects of cypermethrin on interactions of the androgen receptor(AR)with the coactivators androgen receptor-associated protein70(ARA70)and androgen receptor-associated protein 55(ARA55).Results The results showed that AR–ARA70 and AR–ARA55 interactions were remarkably enhanced by dihydrotestosterone(DHT,P≤0.05).Cypermethrin inhibited DHT-induced AR–ARA70 and AR–ARA55 interactions significantly(P≤0.05).Conclusion The study indicates that cypermethrin exhibits inhibitory effects on AR transcription associated with repression of AR–ARA70 and AR–ARA55 interactions in a ligand-dependent manner.The data show novel anti-androgenic mechanisms of cypermethrin that contribute to male reproductive toxicology.Objective The purpose of this study was to investigate the anti-androgenic mechanism of cypermethrin involving coactivators.Methods Mammalian two-hybrid assays were performed to study the effects of cypermethrin on interactions of the androgen receptor(AR) with the coactivators androgen receptor-associated protein70(ARA70) and androgen receptor-associated protein 55(ARA55).Results The results showed that AR–ARA70 and AR–ARA55 interactions were remarkably enhanced by dihydrotestosterone(DHT, P ≤ 0.05). Cypermethrin inhibited DHT-induced AR–ARA70 and AR–ARA55 interactions significantly(P ≤ 0.05).Conclusion The study indicates that cypermethrin exhibits inhibitory effects on AR transcription associated with repression of AR–ARA70 and AR–ARA55 interactions in a ligand-dependent manner. The data show novel anti-androgenic mechanisms of cypermethrin that contribute to male reproductive toxicology.

关 键 词：CYPERMETHRIN ANDROGEN RECEPTOR signaling ANDROGEN receptor-associated PROTEIN 70 ANDROGEN receptor-associated PROTEIN 55 

分 类 号：R114[医药卫生—卫生毒理学]