Urinary metabolomics study of Xiangdan submicron emulsions on a rat model of myocardial ischaemia  

作　　者：Xiuli Wang Xiurong Zhang Tao Gong Zhirong Zhang Laura Weber Xin Wei 

机构地区：[1]School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China [2]Key Laboratory of Drug Targeting and Drug Delivery Systems of Ministry of Education,State Key Laboratory of Biotherapy,Sichuan University,Chengdu 610041,China [3]Pharmaceutical Science,University of Nebraska Medical Center,Omaha 68198-6120,USA

出　　处：《Journal of Traditional Chinese Medical Sciences》2018年第2期139-150,共12页中医科学杂志（英文）

摘　　要：Objective:The aim of this study was to discover the potential biomarkers changes on a rat model of isoproterenol (ISO) induced myocardial ischaemia before and after treatment with a Xiangdan submicron emulsion and to explore the unclearing mechanisms by urinary metabolomics.Methods:Three active traditional Chinese medicine (TCM) ingredients were formulated into a lyophilized submicron emulsion powder with hydroxypropyl-β-cyclodextrin encapsulation by high pressure homogenization technology.The three ingredients were given both separately and combined,and the corresponding effects of the three drugs were compared.Principal component analysis (PCA) and the HPLC-UV analytical method was applied to explore the change of composition of urine metabolites in a rat model of isoproterenol induced myocardial ischaemia.Results:Analytical methods measuring the three active ingredients in urine samples were established.PCA score plots revealed that the therapeutic effect of the Xiangdan submicron emulsion was more significant than that of the Xiangdan injection.There was a synergistic effect among the three active ingredients.Furthermore,studies of rat metabolism changes and structure analysis of biological markers indicated that the mechanism of Xiangdan formulations was related to the inhibition of abnormal tryptophan metabolism.Conclusion:Xiangdan preparations had a confirmed effect in the treatment of coronary heart disease,especially with the novel Xiangdan submicron emulsion formulation.Urinary metabolomics methods to monitor special biomarker changes is an excellent method to reveal the mechanism of action of TCM drugs.

关 键 词：Xiangdan SUBMICRON emulsions URINARY metabolomics BIOMARKER DISCOVERY TRYPTOPHAN DERIVATIVE 

分 类 号：R73[医药卫生—肿瘤]