萘降解细菌的分离及其降解基因的分子检测  

Isolation of naphthalene-degrading bacteria and molecular detection of their naphthalene-catabolic genes

作　　者：李永君[1] 赵化冰[1] 任河山[1] 梁靖[1] 蔡宝立[1] 

机构地区：[1]南开大学微生物学系生物活性材料教育部重点实验室,天津300071

出　　处：《生态学杂志》2006年第7期738-742,共5页Chinese Journal of Ecology

基　　金：国家自然科学基金资助项目(30270274)。

摘　　要：从污水处理厂的活性污泥和石油工业废水中各分离到24个降解萘的细菌分离株,提取这些分离株的总DNA,然后与各种萘降解基因杂交。结果表明,这2个来源的分离株在萘降解基因的种类上有明显不同。来自工业废水的分离株含有萘双加氧酶的铁硫蛋白大亚基基因nahAc,水杨醛脱氢酶基因nahF及其重复基因nahV,水杨酸羟化酶基因nahG及其重复基因nahU,儿茶酚2,3-双加氧酶基因nahH和儿茶酚1,2-双加氧酶基因catA,以及萘趋化蛋白基因nahY。来自活性污泥的分离株只含有nahAc、nahF、nahG和catA,不含有nahY、nahV、nahU和nahH。In this paper,24 naphthalene-degrading bacterial isolates were isolated respectively from wastewater treatment facility's activated sludge and from petroleum wastewater.The total DNA of these isolates were extracted,and hybridized with several naphthalene-catabolic genes.The results indicated that the bacterial isolates isolated from the two sources were quite different in their contained naphthalene-degrading genes.The isolates from petroleum wastewater contained naphthalene dioxygenase Fe-S protein large ...

关 键 词：萘 生物降解 DNA杂交 基因检测 

分 类 号：X172[环境科学与工程—环境科学]