反相高效液相色谱法测定食品中的甜蜜素  

RHPLC determination of sodium cyclamate in food

作　　者：李欣东[1] 张全美[2] 王守卿[1] 

机构地区：[1]淄博市计量测试所,山东淄博255033 [2]淄博市产品质量监督检验所,山东淄博255033

出　　处：《食品与机械》2007年第5期122-124,共3页Food and Machinery

摘　　要：采用反相高效液相色谱法快速测定食品中甜蜜素,在X-terryC18柱上,以甲醇和乙酸铵水溶液(80∶20,V∶V)为流动相进行分离,通过试验得到平均回收率为97.8%,最低检出限为0.4μg/mL的理想效果。经过测定,甜蜜素在0～1.00mg/mL范围内呈良好的线性关系,相关系数为0.9998。This paper determined the content of sodium cyclamate in food by Reverse High Performance Liquid Chromatograph (RHPLC), A mixed solution of methanol in the ratio of 80∶20 (by volume) was used as mobile phase, and X-terry C18 column (5 μm, 4.6 mm×150 mm) was used as the stationary phase. The results was that average recovery was 97.8%,test limit was 0.4 μg/mL. Linearity, 0～1.00 mg/mL, was obtained. Correlation coefficient was 0.999 8.

关 键 词：反相高效液相色谱 甜蜜素 食品 

分 类 号：TS207[轻工技术与工程—食品科学]