The effect of traditional Chinese medicine“Tian Gui Recipe (TGR)”on obesity and anovulation in androgen-sterilized rats(ASR)  

作　　者：Yu Jin(俞瑾) Sun Fei(孙斐) 

机构地区：[1]Obstetrics and Gynecology Hospital, Medical Centre of Fudan University, Shanghai 200011 Obstetrics and Gynecology Hospital, Medical Centre of Fudan University, Shanghai 200011

出　　处：《生殖医学杂志》2002年第z1期13-21,共9页Journal of Reproductive Medicine

基　　金：a grant from the Planning of the Leading Medical Discipline-Gynecology in Integrated Traditional Chinese and Western Medici*ne(No.96001),Shanghai Health Bureau.

摘　　要：Objectives: A traditional Chinese medicine “Tian Gui Recipe (TGR)” has been used to effectively treat clomiphene resistant anovulatory disease and obesity, especially in polycystic ovary syndrome (PCOS) cases with hyperinsulinemia. The effect of TGR on obesity and anovulation was investigated in androgen-sterilized rats (ASR).Methods: Female SD rats at the age of 9 days were divided into 3 groups. Group ASR (n=15): anovulation was demonstrated at the age of 70 days by vaginal smear in rats while 1.25 mg of testosterone propionate was injected subcutaneously on its 9th neonatal day. Group A+H (n=25): TGR were administered to ASR at the age of 80 days for 3 weeks. Rats with regular estrous cycle and ovulation after herbal treatment were included in this group. Group C (n=15): normal ovulated rats were recruited. Around the age of 112 days or on proestrous day, all rats were sacrificed under anesthesia. Serum leptin, testosterone (T), estrogen (E2), follicular stimulating hormone (FSH), luteinizing hormone (LH) levels were measured with radioimmunoassay (RIA). Double immunofluorescent staining combined with confocal laser scanning microscope and dual in situ hybridization were carried out on sections through areas of arcuate nucleus (ARC) to determine whether estrogen receptor (ER) immunoreactivity (IR) or long form of leptin receptor (OB-Rb) mRNA were expressed in neuropeptide Y (NPY) immuno- and mRNA-containing neurons, and their relationship to proopiomelanocortin (POMC) mRNA-containing neurons. Immunohistochemistry and in situ hybridization were used to observe the levels of ER-, NPY-,gonadotrophin releasing hormone (GnRH)-IR and gene expression levels of NPY, OB-Rb, and POMC in ARC. Meanwhile, the criteria of energy state,including daily food intake, retroperitoneal fat depot pad and body weight,were measured and evaluated. Values of immunohistochemistry and in situ hybridization were expressed in mean optic density (MOD).Results:Seventeen out of the 25 rats (68%) in Group A+H were ovulated after herbal treObjectives:A traditional Chinese medicine'Tian Gui Recipe (TGR)'has been used to effectively treat clomiphene resistant anovulatory disease and obesity,especially in polycystic ovary syndrome (PCOS) cases with hyperinsulinemia.The effect of TGR on obesity and anovulation was investigated in androgen-sterilized rats (ASR). Methods:Female SD rats at the age of 9 days were divided into 3 groups.Group ASR (n=15):anovulation was demonstrated at the age of 70 days by vaginal smear in rats while 1.25 mg of testosterone propionate was injected subcutaneously on its 9th neonatal day.Group A+H (n=25).TGR were administered to ASR at the age of 80 days for 3 weeks.Rats with regular estrous cycle and ovulation after herbal treatment were included in this group.Group C (n=15):normal ovulated rats were recruited. Around the age of 112 days or on proestrous day,all rats were sacrificed under anesthe- sia.Serum leptin,testosterone (T),estrogen (E_2),follicular stimulating hormone (FSH),luteinizing hormone (LH) levels were measured with radioimmunoassay (RIA).Double immunofluorescent staining combined with confocal laser scanning mi- croscope and dual in situ hybridization were carried out on sections through areas of ar- cuate nucleus (ARC) to determine whether estrogen receptor (ER) immunoreactivity (IR) or long form of leptin receptor (OB-R_b) mRNA were expressed in neuropeptide Y (NPY) immuno- and mRNA-containing neurons,and their relationship to proopiome- lanocortin (POMC) mRNA-containing neurons.Immunohistochemistry and in situ hybridization were used to observe the levels of ER-,NPY-,gonadotrophin releasing hormone (GnRH)-IR and gene expression levels of NPY,OB-R_b,and POMC in ARC. Meanwhile,the criteria of energy state,including daily food intake,retroperitoneal fat depot pad and body weight,were measured and evaluated.Values of immunohis- tochemistry and in situ hybridization were expressed in mean optic density (MOD). Results:Seventeen out of the 25 rats (68%) in Group A+H were ovulated after herbal treatment.ASR charac

关 键 词：Androgen-sterilized rat Traditional chinese medicine Neuropeptide Y LEPTIN Long form of LEPTIN RECEPTOR PROOPIOMELANOCORTIN ESTROGEN ESTROGEN RECEPTOR GONADOTROPHIN releasing hormone Double immunohistochemistry Dual in situ hybridization mRNA 

分 类 号：R33[医药卫生—人体生理学]