真菌油脂菌种选育与发酵试验研究  

Studies on the strain selection and fermentation for producing fungal lipid

作　　者：林本农 刘飞鹏[2] 林赛芝 

机构地区：[1]福建粮油科学技术研究所,福建福州350002 [2]暨南大学生物工程系,广东广州510632

出　　处：《福州大学学报（自然科学版）》2002年第z1期760-765,共6页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省科技计划项目 (99-Z - 187)

摘　　要：以三株被孢霉作为出发菌株 ,进行紫外线诱变 ,以乙酰水杨酸和SDS作为抑制剂 ,筛选具有抗性的突变株 .通过低温和高糖的筛选 ,再进行摇瓶试验 ,得出最适发酵条件 .通过摇瓶发酵测定各菌株的菌体干重、油脂产量 ,碘价和产不饱和脂肪酸的量 ,最终选出三个优良菌株 :A1- 16产GLA 1.5 3g/L ,ARA 1.16g/L ,DHA 0 .0 9g/L ;A2 - 6产GLA 1.11g/L ;S3- 1产GLA 1.80g/L .Using three strains of Mortierella as original strains, aspirin and SDS as inhibitors, after UV ridiation, resistant mutants were selected. By low temperature and high suger cultivation, screened out high quality strains. The optimal fermentation conditions were decided by flask experiments. Fermentation experiments determinated various strains in DCW, TL, IV and PUFA. Finally, three mutants were selected and their production: A1-16, GLA 1.53?g/L, ARA 1.16?g/L, DHA 0.09?g/L; A2-6, GLA 1.11?g/L; S3-1, GLA 1.80?g/L.

关 键 词：真菌油脂 多不饱和脂肪酸 被孢霉 诱变育种 发酵 

分 类 号：Q819[生物学—生物工程]