Effects of cytokines,growth factors and drugs on matrix metallo proteinases activities of osteoarthritic chondrocytes and synovio cytes  

作　　者：管剑龙 韩星海 施桂英 袁国华 

出　　处：《Journal of Medical Colleges of PLA(China)》2001年第3期200-202,共3页中国人民解放军军医大学学报（英文版）

摘　　要：Objective: To evaluatetheeffectsof somecytokines,TGF-β 1 anddrugson matrixmetalloproteinases(MMPs)activitiesinculturemediumof arthriticchondrocytesandsynoviocytes.Methods:Thechondrocyteandsynoviocytemono-layersisolatedfromthecartilagesandsynovialfluidsin10kneeOA patientsweretreatedwithIL-1βTGF-β 1 ,TNF-α,di-clofenacacid,dexamethasoneor doxycyclineindividuallyandtogetherfor72h.Zymographywasusedto determinetheac-tivitiesof MMP-2and-9.Results:ThechondrocytemonolayersproducedMMP-2and -9,whilethesynoviocytesonlypro-ducedMMP-2.TheMMP-9activitywasmarkedlyenhancedby IL-1βTNF-αanddiclofenac.IL-1βwasthemosteffective stimulus,andhadsynergisticeffectwithTNF-αor diclifenac.MMP-2activitywas notaffected.Doxcycline,TGF-β 1 and dexamethasonecoulddepresstheactivitiesof MMP-9andMMP-2,and antagonizetheenhancingeffectof IL-1βTNF-αor diclofenac.Conclu sion:IL-1βandTNF-αmayplayimportantrolesdegradingOA cartilage,whileTGF-β 1 anddoxycy-clinemaybe protectivefactors.Objective: To evaluatetheeffectsof somecytokines,TGF-β 1 anddrugson matrixmetalloproteinases(MMPs)activitiesinculturemediumof arthriticchondrocytesandsynoviocytes.Methods:Thechondrocyteandsynoviocytemono-layersisolatedfromthecartilagesandsynovialfluidsin10kneeOA patientsweretreatedwithIL-1βTGF-β 1 ,TNF-α,di-clofenacacid,dexamethasoneor doxycyclineindividuallyandtogetherfor72h.Zymographywasusedto determinetheac-tivitiesof MMP-2and-9.Results:ThechondrocytemonolayersproducedMMP-2and -9,whilethesynoviocytesonlypro-ducedMMP-2.TheMMP-9activitywasmarkedlyenhancedby IL-1βTNF-αanddiclofenac.IL-1βwasthemosteffective stimulus,andhadsynergisticeffectwithTNF-αor diclifenac.MMP-2activitywas notaffected.Doxcycline,TGF-β 1 and dexamethasonecoulddepresstheactivitiesof MMP-9andMMP-2,and antagonizetheenhancingeffectof IL-1βTNF-αor diclofenac.Conclu sion:IL-1βandTNF-αmayplayimportantrolesdegradingOA cartilage,whileTGF-β 1 anddoxycy-clinemaybe protectivefactors.

关 键 词：CHONDROCYTES SYNOVIOCYTES MATRIX METALLOPROTEINASES ZYMOGRAPHY 

分 类 号：R965[医药卫生—药理学]