茶色素对肾性高血压诱发左室肥厚的预防作用及其机制研究  

Preventive Effects and Mechanisms of Tea Pigments on Hypertension-induced Cardiac Hypertrophy following Partially Nephrectomy in Rats

作　　者：宋雁[1] 刘兆平[1] 毛伟峰[1] 贾旭东[1] 张晓鹏[1] 王伟[1] 李宁[1] 韩驰[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国预防医学杂志》2009年第2期99-104,共6页Chinese Preventive Medicine

摘　　要：目的研究茶色素对肾性高血压诱发左室肥厚的预防作用及其可能机制。方法雄性SD大鼠通过部分肾切除造成高血压性左室肥厚模型,分别给予含0.10%和0.25%茶色素的水自由饮用,持续4周。同时设阳性对照组(即模型对照组)和阴性对照组(即假手术组)。测定实验前后大鼠的尾动脉收缩压;实验后大鼠心脏和左室重量及血清AngⅡ浓度(RIA法)。心脏组织中转移生长因子-β(TGF-β)、细胞外调节激酶(ERK)、纤维结合蛋白(FN)、胶原蛋白I(collagen I)等蛋白的表达情况。结果模型对照组大鼠心脏重量(1.71±0.23)g,心脏系数0.42±0.06,左心室重量(1.19±0.22)g,左心室系数0.29±0.06,AngⅡ浓度(748.91±154.80)ng/L。经0.10%和0.25%茶色素处理的大鼠心脏重量分别为(1.54±0.13)g和(1.54±0.14)g,心脏系数分别为0.38±0.04和0.39±0.04,左心室重量分别为(1.07±0.11)g和(1.09±0.11)g,AngⅡ浓度分别为(466.18±111.44)mg/L和(537.55±61.09)ng/L,与阳性对照组比较差异均有统计学意义(P<0.05)。阳性对照组ERK、TGF-β、FN、collagenⅠ蛋白质表达水平较阴性对照组显著增高;0.25%茶色素组ERK、TGF-β、FN、collagenⅠ蛋白质表达水平与阳性对照组比较呈显著降低。结论茶色素能有效预防部分肾切除所引起的左室肥厚,这种作用与茶色素对AngⅡ、TGF-β、FN和collagenⅠ等物质的调节有关,而且茶色素可能对ERK信号转导通路具有一定的抑制作用。Objective To investigate the effect of tea pigments on hypertension -induced cardiac hypertrophy following partially nephrectomy in rats and its potential mechanisms. Methods Animal models bearing cardiac hypertrophy were replicated in male SD rats following partially nephrectomy (PNX).0.10% or 0.25% tea pigments was administered to rats by drinking, respectively, for 4 weeks. PNX control and sham-operation control (SHAM) were simultaneously established.Systolic pressure of rats was measured through tail at...

关 键 词：茶色素 左室肥厚 血管紧张素Ⅱ 转移生长因子-Β 细胞外调节激酶 

分 类 号：R541.3[医药卫生—心血管疾病]