2种方法测定稻米蛋白质含量及其相关性分析  

The crude protein contents in rice grain measured by two different methods and their relationship

作　　者：周丽慧[1] 刘巧泉[1] 张昌泉[1] 徐勇[1] 汤述翥[1] 顾铭洪[1] 

机构地区：[1]扬州大学教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2009年第1期68-72,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金重点资助项目(30530470);国家高技术研究发展计划项目(863-2006AA10A102);教育部"新世纪优秀人才支持计划"项目(NCET-07-0736);江苏省自然科学基金资助项目(BK2007510)

摘　　要：以483份稻米样品为对象,分别用近红外光谱技术(NIRS)和凯氏定氮法测定糙米和精米中的蛋白质含量。通过同一样品NIRS多次测定、同一样品NIRS测定结果与凯氏定氮法测定结果的比较分析,证实NIRS在测定稻米蛋白质含量时具有很好的稳定性和准确性;分析了2种方法不同测定值之间的相关性,建立了相应的回归方程,并讨论了基于NIRS技术预测不同类型稻米中蛋白质含量的可靠性,以求为品质育种提供相对准确的数据。Using collected 483 rice grain samples,the crude protein contents in brown and milled rice were carefully determined by either near infrared spectroscopy(NIRS) and Kjeldahl nitrogen determination method.The comparison of data in the same sample by NIRS and of data by two methods demonstrated that it was of fine stability and veracity by the NIRS to measure the crude protein contents.The results of regression analysis showed that there was a significant linear correlationship between the crude protein conten...

关 键 词：水稻 种子 蛋白质含量 近红外光谱技术 凯氏定氮法 

分 类 号：S511[农业科学—作物学]