STUDIES ON BLOOD GLUTATHIONE PEROXIDASE PROTEIN LEVEL OF CHILDREN IN KESHAN DISEASE AND KASHIN-BECK DISEASE AREAS  

作　　者：Tan Wuhong(谭武红) Zhong Xiaohong(种晓红) Yang Zhantian(杨占田) Zhai Lianbang(翟连榜) Wang Lixin(王立新) Xu Guanglu(徐光禄) 

机构地区：[1]Institute of Endemic Diseases, Medical School of Xi''an Jiaotong University, Xi''an 710061, China. Institute of Endemic Diseases, Medical School of Xi''an Jiaotong University, Xi''an 710061, China. Institute of Endemic Diseases, Medical School of Xi''an Jiaotong University, Xi''an 710061, China. Institute of Endemic Diseases, Medical School of Xi''an Jiaotong University, Xi''an 710061, China. Institute of Endemic Diseases, Medical School of Xi''an Jiaotong University, Xi''an 710061, China. Institute of Endemic Diseases, Medical School of Xi''an Jiaotong University, Xi''an 710061, China.

出　　处：《Academic Journal of Xi'an Jiaotong University》2002年第2期133-135,共3页西安交通大学学报（英文版）

摘　　要：Objective To oberve the change in blood glutathione peroxidase (GSH-Px) protein levels of residents in the low-selenium (Se) area by contrasting the blood GSH-Px protein level of the children in the Keshan disease area with those in the Kashin-Beck disease and non-endemic areas. Methods GSH-Px protein levels were measured by enzyme-linked immunosorbent assays (ELISA). The Se content and GSH-Px activity were assayed by the 2,3-diaminonaphthalene spectrofluorimetric method and glutathione reductase-coupled method respectively. Results ①The blood Se content and GSH-Px protein level of children in Keshan disease area (Moding) were significantly lower than those in Xi’an non-endemic area, however, there was no significant difference when compared with the low-Se non-endemic area; ②The blood Se content, GSH-Px activity and GSH-Px protein level of children in the Kashin-Beck disease area (Yulin) were significantly lower than those of children in two non-endemic areas and in the Keshan disease area; ③The blood Se content and GSH-Px activity were positively correlated to the GSH-Px protein level respectively. Conclusion These results indicate that the blood GSH-Px protein level is decreased in the low-Se residents. The Se status not only affects the GSH-Px activity but also regulate the GSH-Px protein level.Objective To oberve the change in blood glutathione peroxidase (GSH-Px) protein levels of residents in the low-selenium (Se) area by contrasting the blood GSH-Px protein level of the children in the Keshan disease area with those in the Kashin-Beck disease and non-endemic areas. Methods GSH-Px protein levels were measured by enzyme-linked immunosorbent assays (ELISA). The Se content and GSH-Px activity were assayed by the 2,3-diaminonaphthalene spectrofluorimetric method and glutathione reductase-coupled method respectively. Results ①The blood Se content and GSH-Px protein level of children in Keshan disease area (Moding) were significantly lower than those in Xi'an non-endemic area, however, there was no significant difference when compared with the low-Se non-endemic area; ②The blood Se content, GSH-Px activity and GSH-Px protein level of children in the Kashin-Beck disease area (Yulin) were significantly lower than those of children in two non-endemic areas and in the Keshan disease area; ③The blood Se content and GSH-Px activity were positively correlated to the GSH-Px protein level respectively. Conclusion These results indicate that the blood GSH-Px protein level is decreased in the low-Se residents. The Se status not only affects the GSH-Px activity but also regulate the GSH-Px protein level.

关 键 词：Kashan DISEASE Kashin-Beck DISEASE CHILDREN GLUTATHIONE PEROXIDASE PROTEIN 

分 类 号：Q503[生物学—生物化学]