柱前衍生高效液相色谱法测定乳制品中甘氨酸的含量  

Determination of glycin in dairy products by pre-column derivatization HPLC

作　　者：赵志红[1] 张逢秋[1] 朱慧[1] 施文蓉[1] 

机构地区：[1]杭州娃哈哈集团有限公司研发中心,杭州310018

出　　处：《中国食品添加剂》2009年第4期169-172,共4页China Food Additives

摘　　要：采用单磺酰氯柱前衍生高效液相色谱法测定乳制品中甘氨酸的含量。应用单磺酰氯作为柱前衍生试剂,55℃温度下,反应15min。衍生产物用C18柱(3.9mm×150mm,5μm)分离,0.1mol/L醋酸钠(pH7.2):乙腈75:25为流动相进行洗脱,流速1.0mL/min,在330nm的波长下,能准确检测出乳制品中游离甘氨酸。方法回收率在98.2%～102%之间,在0～205mg/L范围内,线性方程相关系数为0.99994,相对标准偏差RSD为0.87%。该方法衍生反应简单,时间短。衍生产物在330nm具有较强的紫外吸收,且产物单一稳定,冰箱中存放可稳定一星期以上,分析速度快,7min内可与其他峰完全分离。本方法具有操作简单?结果重复性好?准确等优点。The glycin in dairy products was determined by dansyl chloride as the pre-column derivation reagent at 55℃ for 15min.The derivation can be detected easily and accurately by HPLC.The pre-column derivation was separated by C18 column(3.9 mm × 150mm,5μm),and eluted by mobile phase of 0.1mol/L sodium acetate(pH7.2)and acetonitrile with proportion of 75:25,detected at 330nm wavelength.In 7minutes,the derivation was effectively separated.The recovery was between 98.2%～102%,the correlation was 0.99994 with the range between 0～205mg/L.The derivation was a single product and had strong UV absorption at 330nm.It was stable for more than one week in the refrigerator.The method had the advantage of simple operation,good repetition and accuracy.

关 键 词：甘氨酸 单磺酰氯 柱前衍生色谱法 乳制品 

分 类 号：TS207.3[轻工技术与工程—食品科学]