OK-432 INHIBITED B16 MELANOMA GROWTH AND INDUCED A TH1 DOMINANT STATE IN TUMOR BEARING MOUSE  

作　　者：王湘辉 藤本敏博 张滨 磨伊正义 柴福录 

机构地区：[1]Department of the General Surgery, Lanzhou General Hospital of PLA, Lanzhou 730050, China [2]Cancer Research Institute of Kanazawa University, Japan

出　　处：《Chinese Journal of Cancer Research》2002年第1期33-36,共4页中国癌症研究（英文版）

基　　金：This work was supported in part by the Japan China Sasakawa Medical Fellowship.

摘　　要：Objective: To investigate the antitumor mechanisms of the streptococcal preparation OK-432. Methods: Using C57BL/6 mouse bearing B16 melanoma, we observed the antitumor activity of OK-432 and investigated the effect of OK-432 on multi-cytokine (IL-2, IL-4, IL-6, IL-10, IL-12, IFN-γ) production of mouse splenocyte both in vitro and in vivo. Results: As compared with control, OK-432 significantly inhibited B16 melanoma growth and lengthened mice survival time (P<0.05). In vitro OK-432 could stimulate splenocyte from tumor bearing mice to secrete IL-6, IL-12, IFN-γ and IL-10 remarkably (P<0.01). In vivo OK-432 led to the increased production of IL-2, IL-12 and IFN-γ but decreased production of IL-10 (P<0.05). When the splenocytes harvested from OK-432 treated mice were stimulated with OK-432 again in vitro, the production of IFN-γ increased and IL-10 decreased significantly (P<0.05). Conclusion: OK-432 could boost multiple cytokines production, especially IL-12 which skewed T cells in a Th1 dominant state and enhanced the host antitumor activities.Objective: To investigate the antitumor mechanisms of the streptococcal preparation OK-432. Methods: Using C57BL/6 mouse bearing B16 melanoma, we observed the antitumor activity of OK-432 and investigated the effect of OK-432 on multi-cytokine (IL-2, IL-4, IL-6, IL-10, IL-12, IFN-γ) production of mouse splenocyte both in vitro and in vivo. Results: As compared with control, OK-432 significantly inhibited B16 melanoma growth and lengthened mice survival time (P<0.05). In vitro OK-432 could stimulate splenocyte from tumor bearing mice to secrete IL-6, IL-12, IFN-γ and IL-10 remarkably (P<0.01). In vivo OK-432 led to the increased production of IL-2, IL-12 and IFN-γ but decreased production of IL-10 (P<0.05). When the splenocytes harvested from OK-432 treated mice were stimulated with OK-432 again in vitro, the production of IFN-γ increased and IL-10 decreased significantly (P<0.05). Conclusion: OK-432 could boost multiple cytokines production, especially IL-12 which skewed T cells in a Th1 dominant state and enhanced the host antitumor activities.

关 键 词：Streptococcal preparation INTERLEUKIN-12 CYTOKINE Immunotherapy 

分 类 号：R730.2[医药卫生—肿瘤]