抗氧化能力的体外测定方法研究进展  

Progress of Analytical Methods for Antioxidant Capacity in Vitro

作　　者：王晓宇[1] 杜国荣[2] 李华[3] 

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710062 [2]西安文理学院生命科学系,陕西西安710065 [3]西北农林科技大学葡萄酒学院,陕西杨凌712100

出　　处：《食品与生物技术学报》2012年第3期247-252,共6页Journal of Food Science and Biotechnology

基　　金：中央高校基本科研业务费专项资金项目(GK201002003);陕西师范大学青年基金项目(200901003)

摘　　要：水果、蔬菜、茶叶和红葡萄酒等食品被认为对人体健康具有积极的作用,其主要原因在于这些产品中的一些天然产物具有抗氧化作用。随着对天然抗氧化物质的关注程度不断提高,研究者开发出许多种体外抗氧化能力测定方法。常用的体外抗氧化测定方法有:总氧自由基清除能力法、还原能力测定法、ABTS自由基清除能力法、DPPH自由基清除能力法、羟自由基清除能力法、超氧自由基清除能力法和脂质过氧化法等。这些方法的反应原理和反应环境各不相同。作者系统地介绍了体外各种抗氧化能力测定方法,分析了这些方法的原理、特点和优缺点,并且对现行方法在实际中的应用现状和研究动态进行了评述。Due to high antioxidant activity,many foodstuffs and beverages such as fruits,vegetables,tea,and red wine were exhibited the excellent beneficial influence on human health.With increasing interest in the function and diversity of antioxidants in foods,several in vitro methods for measuring antioxidant activity of food,beverages and biological samples have been developed.The most commonly assay methods include oxygen radical absorbance capacity(ORAC),reducing power,determination of total phenols,2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid)(ABTS),2,2-diphenyl-1-picrylhydrazyl(DPPH),hydroxyl radical-scavenger activity,superoxide radical-scavenger activity and lipid peroxidation inhibition.These methods differ in terms of their assay principles and experimental conditions.This review describes the methodology for assessing in vitro antioxidant capacity,including the principles,characteristics,merits and drawbacks of every method.In addition,their current status and problems in practical application are critically evaluated.

关 键 词：抗氧化能力 体外 分析方法 

分 类 号：R151[医药卫生—营养与食品卫生学]