乳中掺尿素快速检测方法影响因素的探索  

Study on the Influencing Factors for Rapid Detection Methods of Urea Adulteration in Milk

作　　者：买热木尼沙.吾甫尔 秦菊[2] 张晓红[2] 陈雪莲[2] 王志琴[2] 

机构地区：[1]新疆巴音郭楞蒙古自治州动物疾病控制与诊断中心,新疆库尔勒841000 [2]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2012年第7期1315-1320,共6页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区"十一五"科技重大专项(200731134-3);新疆维吾尔自治区重点学科基础兽医学;新疆维吾尔自治区动物生物技术重点开放实验室

摘　　要：[目的]部分不法奶商在原料乳中加水后同时加尿素来提高蛋白质含量及增加密度,快速检测乳中掺尿素,找出并拒收这部分掺假异常乳。[方法]对牛乳掺尿素的检测方法(格里斯法)所用的试剂浓度、用量、搭配比例进行筛选,对掺不同浓度尿素的牛乳采用试管法进行检测比较。[结果]用1%亚硝酸钠溶液0.4mL的剂量,格里斯试剂用量为0.2 g,浓硫酸用量为1 mL,对掺尿素乳检测的结果比较明显,尿素最低检出量为0.04%,能方便、快速的检测出牛乳掺尿素。[结论]该法用于牛乳掺尿素的检测,操作简单,检测快速,灵敏度较高,检测成本低,适用于各奶站及牛乳检测机构的快速检验。[Objective]The aim of this study was to identify and reject the adulteration and abnormal raw milk in which water as well as urea was added to increase the protein content and density by some of the illegal milk suppliers.[Method]The present study selected the reagent concentration,dosage,and mix proportion in detecting urea adulteration in milk(Griess method).With test - tube detection method of different concentrations of urea - milk was compared.[Result]The results showed that 1%0.4 mL dose of sodium nitrite solution,griess reagent usage for 0.2 g,amount of concentrated sulfuric acid is 1ml,milk urea - doped test results was more obvious The minimum detected quantity of urea is 0.04%.It is convenient and quick to detect milk urea - doped.[Conclusion]The results proved that the sensitivity of this new quick - test method is high,convenient,and cost - saving.It can be applied at milk collection station and milk detection agencies as well.

关 键 词：牛乳 尿素 格里斯试剂 快速检测 

分 类 号：S879.1[农业科学—畜牧兽医] TS252.7[轻工技术与工程—农产品加工及贮藏工程]