沙门氏菌的依赖于核酸序列恒温扩增检测方法的建立  

Establishment of the nucleic acid sequence-based amplification method of detecting Salmonella

作　　者：雷质文[1] 姜英辉[1] 王妍婷[1] 赵丽青[1] 张健[1] 倪鑫[2] 王建广[1] 梁成珠[1] 

机构地区：[1]山东出入境检验检疫局技术中心,青岛266002 [2]中国海洋大学,青岛266003

出　　处：《食品安全质量检测学报》2011年第5期248-252,共5页Journal of Food Safety and Quality

基　　金：国家质检总局科研项目(2009IK170)资助项目

摘　　要：采用自行建立和优化的依赖于核酸序列恒温扩增(nucleic acid sequence-based amplification,NASBA)检测体系,对沙门氏菌进行检测。采用沙门氏菌的invA基因为目的片段设计特异性引物,建成可快速检测沙门氏菌的NAS-BA检测法,并进行了特异性和灵敏度试验。结果表明:所建立起的NASBA检测方法,灵敏度为7.1×102cfu/ml,高于普通PCR方法。A new method,which based on Nucleic Acid Sequence-based Amplification(NASBA) to detect Salmonella of sample,was established.A specific set of primers was synthesized to target the invA gene of Salmonella so as to establish Nucleic Acid Sequence-based Amplification method.Specific and sensitivity were tested.The results shows that the sensitivity of NASBA was 7.1×102 cfu/ml which was higher than the result of PCR method.Detecting Salmonella with NASBA was more specific and sensitive than PCR method and has lower instrumental requirement.

关 键 词：沙门氏菌 检测 依赖于核酸序列恒温扩增 

分 类 号：R155.5[医药卫生—营养与食品卫生学]