固相萃取高效液相色谱荧光检测法联合测定食品中苯并(a)芘  

Determination of benzopyrene in food by solid phase extraction-high performance liquid chromatography with fluorescent detector

作　　者：吴敏[1] 李政[2] 黎翠玉[1] 杨黎忠[1] 徐敦明[1] 周昱[1] 

机构地区：[1]厦门出入境检验检疫局,厦门361026 [2]江西农业大学食品科学与工程学院,南昌330045

出　　处：《食品安全质量检测学报》2012年第2期82-88,共7页Journal of Food Safety and Quality

基　　金：国家质检总局科研计划资助项目(2012IK192)

摘　　要：目的采用固相萃取高效液相色谱荧光法(HPLC-FLD)联合测定食品中苯并(a)芘的含量。方法根据不同样品基质对样品提取条件进行了优化,采用固相萃取柱净化,XbridgeTMC18色谱柱分离,外标法定量。结果苯并(a)芘在0~100 ng/mL浓度范围内线性关系良好,相关系数r=0.9994。本方法的测定低限(S/N≥10):茶油、罗非鱼和猪肉为1.0μg/kg,大米、包菜、茶叶、饮用水和橙汁为0.5μg/kg;添加浓度为0.5μg/kg^100μg/kg时,回收率范围为77.3%~93.2%,变异系数(n≥6)为0.65%~7.18%。结论该方法前处理简单,分析时间短,具有良好的灵敏度和准确性,适用于各类食品中苯并(a)芘的测定。Objective A method of solid phase extraction-high performance liquid chromatography with fluorescent detector(HPLC-FLD)was developed for determination of benzopyrene(BaP) in foodstuffs.Method The analytical procedure includes optimization of extraction conditions,purification using a SPE column and separation by XbridgeTMC18Chromatographic column,followed by a quantitative external standard method.Results The method showed a good linearity over the range of 0~100 ng/mL for benzopyrene(BaP) with r=0.9994.The detection limits(S/N≥10) were 1.0 μg/kg for tea seed oil,tilapia,pork and 0.5 μg/kg for rice,cabbage,tea,water and orange juice.The recoveries were 77.3%~93.2% at spiked levels of 0.5 μg/kg^100 μg/kg and the relative standard deviations were 0.65%~7.18%(n≥6).Conclusion This method has the advantages of easy to operate,fast to perform,with high sensitivity and accuracy,and it was suitable for the detection of benzopyrene in foodstuffs.

关 键 词：苯并(A)芘 食品 高效液相色谱-荧光法 固相萃取 

分 类 号：R155.5[医药卫生—营养与食品卫生学]