HPLC-DAD法同时测定高脂肪食品中黄色2G、奶油黄、柑橘红2和苏丹红Ⅰ～Ⅳ  

HPLC-DAD simultaneous determination of yellow 2G, butter yellow, citrus red 2 and sudan red Ⅰ～Ⅱ in high fat foods

作　　者：奚星林[1] 王岚[1] 李荀[1] 李双[1] 何吉子 邹志飞[1] 

机构地区：[1]广东出入境检验检疫局检验检疫技术中心,广州510623

出　　处：《食品安全质量检测学报》2013年第1期245-250,共6页Journal of Food Safety and Quality

基　　金：中国检验检疫科学院基本科研业务费专项基金资助项目(2009JK011);广东省科技基础条件建设项目(粤科财字[2008]658;[2010]185);"十二五"农村领域国家科技计划课题(2011BAD23B05-4);国家质检总局公益性行业科研专项(201110019)~~

摘　　要：目的建立同时测定高脂肪食品中黄色2G、奶油黄、柑橘红2和苏丹红Ⅰ～Ⅳ的HPLC-DAD法。方法样品经环已烷/乙酸乙酯(1∶1,v/v)萃取,Bio-BeadsSX3凝胶层析柱净化去除油脂、天然色素等大分子干扰物质。采用C18色谱柱,以甲醇和酸性水溶液(pH4.0)为流动相梯度洗脱,二极管阵列检测器多波长同时检测这七种色素。结果在1.0～10.0mg/L范围内,峰面积与进样量呈良好的线性关系,线性相关系数大于0.999,回收率在81.0%~98.2%,相对标准偏差在0.89%~5.21%。黄色2G、奶油黄、柑橘红2的检出限均达到1.0mg/kg,苏丹红Ⅰ～Ⅳ色素的检出限均达到2.0mg/kg。结论该方法简便、快捷、灵敏、准确、重现性好,能满足相关色素同时检测的需要。Objective To establish an HPLC-DAD method for simultaneous determination of yellow 2G, butter yellow, citrus red 2 and sudan red I^IV. Methods Samples were extracted directly with a mixture of cyclohexane-athylacetate (1∶1, v/v ) and purified by a Bio-beads SX3 gel column, to remove interfering high molecular fatty substances and natural pigments in food samples. HPLC was carried out on a C18 column with MeOH+H2O (pH 4.0) as mobile phase in the gradient elution. Multiwavelength diode array detection (DAD) was applied in the determination of the 7 kinds of pigments. Results Good linear relationships in the range of 1.0~10 mg/L of 7 compounds were obtained with correlation coefficients (r) all above 0.999. The RSDs were in the range of 0.89%~5.21%, and average recoveries were 81.0%~98.2%. The detection limits were 1.0 mg/kg for yellow 2G, butter yellow, citrus red 2 and 2.0 mg/kg for sudan red I^IV, respectively. Conclusion The established method is simple, rapid, sensitive, accurate and repeatable, and can be used for the simultaneous determination of relevant pigments.

关 键 词：凝胶渗透色谱净化 高压液相色谱法 黄色2G 奶油黄 柑橘红2 苏丹红 高脂肪食品 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]