衍生化法测定山药中还原型谷胱甘肽(GSH)和总巯基(-SH)含量  

Determination of reduced glutathione (GSH) and total thiols (-SH) in Rhizoma Dioscoreae by derivatization methods

作　　者：金京实[1] 沈丽[1] 崔胜云[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,延吉133002

出　　处：《食品安全质量检测学报》2013年第4期1155-1160,共6页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(21165021)~~

摘　　要：目的用衍生化法测定山药中还原型谷胱甘肽(GSH)和总巯基(-SH)的含量。方法利用4,4′-二硫代联吡啶(4-DPS)和邻苯二甲醛(OPA)为衍生化试剂,采用细胞破壁和衍生化为一体的样品前处理方法,分别用UV-vis分光光度法和荧光光谱法测定了山药鲜品中总巯基(-SH)、GSH的含量。结果山药中含较丰富的总巯基和GSH,含量分别达0.4556μmol/g和0.1792μmol/g。UV-vis分光光度法和荧光光谱法测定方法的检测限分别为1.0458μmol/L和0.5666μmol/L,回收率分别为98.73%和98.40%。结论本研究对山药中巯基化合物的含量、测定方法学和样品前处理方法提供了重要的基础数据和参考依据。Objective To detect reduced glutathione (GSH) and total thiols (-SH) in Rhizoma Dioscoreae by derivatization methods. Methods Total thiols and reduced glutathione in Rhizoma Dioscoreae were de-termined by UV-vis spectrophotometry and fluorophotometry using 4,4′-dithiodipyridine (4-DPS) and o-phthaldehyde (OPA) as derivatization agents. Samples were prepared by simultaneous derivatization during cell rupture, Results 0.4556 μmol/g of total thiols and 0.1792 μmol/g of GSH were detected in the sample . The detection limits for UV-vis spectrophotometry and fluorophotometry were 1.0458 μmol/L and 0.5666 μmol/L, and recoveries were 98.73%and 98.40%, respectively. Conclusion This study provides useful data and references for the content of thiol compounds in Rhizoma Dioscore determination methodology and pre-treatment methods for plant sample analysis.

关 键 词：山药 还原型谷胱甘肽 4 4′-二硫代联吡啶 邻苯二甲醛 衍生化 

分 类 号：R155.5[医药卫生—营养与食品卫生学]