家蚕丝素固定化木瓜蛋白酶的研究  

Immobilization of papain and its application to silk peptide production

作　　者：陈芳艳[1] 纪平雄[2] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]华南农业大学理学院,广东广州510642

出　　处：《华南农业大学学报》2004年第3期83-86,共4页Journal of South China Agricultural University

基　　金：华南农业大学校长基金资助项目(5400-K00014)

摘　　要：采用质量分数为5％戊二醛在常温下处理脱胶丝素40 min,洗净、真空干燥,制备活化丝素.以活化丝素为载体,采用共价交联法制备固定化木瓜蛋白酶.研究结果表明,最佳的固定化条件为:给酶量0.36mg/g(蛋白质质量浓度为0.12 mg/mL)、pH 7.5、温度4℃左右条件下,固定6 h,所得到的固定化酶活力为1 786.93 U/g,活力回收高达69.54％.The degummed fibroin was treated with w=0.5% glutaraldehyde at normal temperature for 40 min and got the activated fibroin. Using the activated fibroin as carrier, the papain was immobilized by covalence-crosslinking method. The result showed that the optimum conditions for immobilization were: the enzyme loading amount was 0.36 mg/g silk fibroin; the temperature was 4 ℃ ; the immobilizing time was 6 h; and the pH value was 7.5. The activity and activity recovery of the immobilized papain were 1 786.93 U/g and 69.54% , respectively.

关 键 词：家蚕丝素 固定化酶 木瓜蛋白酶 共价交联法 

分 类 号：Q556.3[生物学—生物化学]