Inhibition of Pim-1 attenuates the proliferation and migration in nasopharyngeal carcinoma cells  

作　　者：Wei Jie Qi-Yi He Bo-Tao Luo Shao-Jiang Zheng Yue-Qiong Kong Han-Guo Jiang Ru-Jia Li Jun-Li Guo Zhi-Hua Shen 

机构地区：[1]Department of Pathology & Pathophysiology,School of Basic Medicine Science,Guangdong Medical College [2]Hainan Provincial Key Laboratory of Tropical Medicine,Hainan Medical College

出　　处：《Asian Pacific Journal of Tropical Medicine》2012年第8期645-650,共6页亚太热带医药杂志（英文版）

基　　金：supported by grants from the Doctoral Program of Guangdong Medical College(B2010013);National Natural Science Foundation of China(81000073);Natural Foundation of Hainan Province of China 1811197, 310043,and 811201)

摘　　要：Objective:To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma(NPC) cells.Methods:Pim-1 expressions in NPC cell lines CNE1,CNE1-GL,CNE-2Z and C666-1 were examined by KT-PCR,western blotting and immunoflucesence,respectively.After CNE1,CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor,quercelagetin,the cell viability,colony formation rate and migration ability were analyzed.Results:Pim-1 expression was negative in well-differentiated CNE1 cells,whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells.Interestingly,CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1.Treatment of CNE1-GL and C666-1 cells with quercelagetin significantly decreased the cell viability,colony formation rate and migration ability but not the CNE1 cells.Conclusions:These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration,and targeting Pim-1 may be a potential treatment for anti-Pim-1-expressed NPCs.Objective:To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma(NPC) cells.Methods:Pim-1 expressions in NPC cell lines CNE1,CNE1-GL,CNE-2Z and C666-1 were examined by KT-PCR,western blotting and immunoflucesence,respectively.After CNE1,CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor,quercelagetin,the cell viability,colony formation rate and migration ability were analyzed.Results:Pim-1 expression was negative in well-differentiated CNE1 cells,whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells.Interestingly,CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1.Treatment of CNE1-GL and C666-1 cells with quercelagetin significantly decreased the cell viability,colony formation rate and migration ability but not the CNE1 cells.Conclusions:These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration,and targeting Pim-1 may be a potential treatment for anti-Pim-1-expressed NPCs.

关 键 词：NASOPHARYNGEAL CARCINOMA PIM-1 Quercetagetin CELL PROLIFERATION CELL MIGRATION 

分 类 号：R739.63[医药卫生—肿瘤]