Efficacy of boswellic acid on lysosomal acid hydrolases,lipid peroxidation and anti-oxidant status in gouty arthritic mice  

作　　者：Evan Prince Sabina Haridas Indu Mahaboobkhan Rasool 

机构地区：[1]School of Bio Sciences and Technology,VIT University Vellore-632 014

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2012年第2期128-133,共6页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To evaluate the efficacy of boswellic acid against monosodium urate crystal-induced inflammation in mice.Methods:The mice were divided into four experimental groups.GroupⅠserved as control;mice in groupⅡwere injected with monosodium urate crystal;groupⅢconsisted of monosodium urate crystal-induced mice who were treated with boswellic acid(30mg/kg/b.w.);groupⅣcomprised monosodium urate crystal-induced mice who were treated with indomethacin(3mg/kg/b.w.).Paw volume and levels/activities of lysosomal enzymes,lipid peroxidation,anti-oxidant status and inflammatory mediator TNF-αwere determined in control and monosodium urate crystal-induced mice.In addition,the levels ofβ-glucuronidase and lactate dehydrogenase were also measured in monosodium urate crystal-incubated polymorphonuclear leucocytes(PMNL)in vitro.Results:The activities of lysosomal enzymes,lipid peroxidation,and tumour necrosis factor-αlevels and paw volume were increased significantly in monosodium urate crystal-induced mice,whereas the activities of antioxidant status were in turn decreased.However,these changes were modulated to near normal levels upon boswellic acid administration.In vitro,boswellic acid reduced the level ofβ-glucuronidase and lactate dehydrogenase in monosodium urate crystal-incubated PMNL in concentration dependent manner when compared with control cells.Conclusions:The results obtained in this study further strengthen the anti-inflammatory/antiarthritic effect of boswellic acid,which was already well established by several investigators.Objective:To evaluate the efficacy of hoswellic acid against monosodium urate crystal-induced inflammation in mice.Methods:The mice were divided into lour experimental groups.Group1 served as control:mice in group Ⅱ were injected with monosodiuni urate crystal;group Ⅲconsisted of monosodium urate crystal-induced mice who were treated with hoswellic acid(30mg/kg/b.w.);group Ⅳ comprised monosodium urate crystal-induced mice who were treated with indomethacin(3 mg/kg/b.w.)-Paw volume and levels/activities of lysosomal enzymes,lipid peroxidation,anti-oxidant status and inflammatory mediator TNF-α were determined in control and monosodiuni urate crystal-induced mice.In addition,the levels of β-glucuronidase and lactate dehydrogenase were also measured in monosodium urate crystal-incubated polymorphonuclear leucocytes(PMNL)in vitro.Results:The aclivities of lysosomal enzymes,lipid peroxidation,and tumour necrosis factor-α levels and paw volume were increased significantly in monosodium urate crystal-induced mice,whereas the activities of antioxidant status were in turn decreased.However,these changes were modulated to near normal levels upon hoswellic acid administration.In ritro,hoswellic acid reduced the level of β-glucuronidase and lactate dehydrogenase in monosodium urate crystal-incubated PMNL in concentration dependent manner when compared with control cells.Conclusions:The results obtained in this study further strengthen the anti-inflammaton/antiarthritic effect of hoswellic acid,which was aheady well established by several investigators.

关 键 词：Boswellic ACID GOUTY arthritis Indomethacin LYSOSOMAL enzymes POLYMORPHONUCLEAR LEUCOCYTES Monosodium urate Lipid peroxidation Antioxidant STATUS Anti-inflammatory effect Antiarthritic effect Inflammation 

分 类 号：R285[医药卫生—中药学]