Protective effects of naringenin eye drops on N-methylN-nitrosourea-induced photoreceptor cell death in rats  

作　　者：Jun-Li Lin Yan-Dong Wang Yan Ma Chun-Mei Zhong Mei-Rong Zhu Wen-Pei Chen Bao-Qin Lin 

机构地区：[1]School of Chinese Materia Medica, Guangzhou University of Chinese Medicine [2]Zhongshan Ophthalmic Center, Sun Yat-Sen University [3]Zhuhai United Laboratories Co., Ltd

出　　处：《International Journal of Ophthalmology(English edition)》2014年第3期391-396,共6页国际眼科杂志（英文版）

基　　金：Supported by Guangdong Provincial Department of Science and Technology Grant(No.2011B031700052)

摘　　要：AIM:To investigate the effects of naringenin eye drops on N-methyl-N-nitrosourea (MNU)-induced photoreceptor cell death in rats.METHODS:Photoreceptor cell death was induced by single intraperitoneal injection of MNU(60 mg/kg)in rats.Both eyes of all animals were instilled with one drop of vehicle,0.5% or 1.0% naringenin eye drops three times per day from 7d before to 17d after MNU injection.Effects of naringenin on MNU-induced photoreceptor cell death were evaluated by electrophysiological and histological analysis.RESULTS:Flash electroretinography (FERG)and oscillatory potentials (OPs) recordings showed that the vehicle control group had remarkable reduction of amplitudes and prolongation of latency times.FERG and OPs responses were significantly reversed in MNUinduced rats treated with 0.5%or 1.0% naringenin eye drops compared with the vehicle control.The retinal morphological results showed that naringenin dosedependently preserved the outer nuclear layer,outer retina and total retina.CONCLUSION:These results indicate that topical treatment with naringenin eye drops prevented retinal neurons from MNU-induced structural and functional damages.AIM:To investigate the effects of naringenin eye drops on N-methyl-N-nitrosourea (MNU)-induced photoreceptor cell death in rats.METHODS:Photoreceptor cell death was induced by single intraperitoneal injection of MNU(60 mg/kg)in rats.Both eyes of all animals were instilled with one drop of vehicle,0.5% or 1.0% naringenin eye drops three times per day from 7d before to 17d after MNU injection.Effects of naringenin on MNU-induced photoreceptor cell death were evaluated by electrophysiological and histological analysis.RESULTS:Flash electroretinography (FERG)and oscillatory potentials (OPs) recordings showed that the vehicle control group had remarkable reduction of amplitudes and prolongation of latency times.FERG and OPs responses were significantly reversed in MNUinduced rats treated with 0.5%or 1.0% naringenin eye drops compared with the vehicle control.The retinal morphological results showed that naringenin dosedependently preserved the outer nuclear layer,outer retina and total retina.CONCLUSION:These results indicate that topical treatment with naringenin eye drops prevented retinal neurons from MNU-induced structural and functional damages.

关 键 词：NARINGENIN N-METHYL-N-NITROSOUREA photoreceptor cell death retinitis pigmentosa 

分 类 号：R774.1[医药卫生—眼科]