Protective effect of saturated hydrogen saline against blue light-induced retinal damage in rats  

作　　者：Mei Feng Xing-Hua Wang Xiao-Bo Yang Qing Xiao and Fa-Gang Jiang 

机构地区：[1]Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China

出　　处：《International Journal of Ophthalmology(English edition)》2012年第2期151-157,共7页国际眼科杂志（英文版）

摘　　要：AIM: To explore the effect of saturated hydrogen saline on blue light-induced retinal damage in rats. METHODS: The retinal damage of rats was induced by blue light exposure for 6 hours and examined 8 hours, 16 hours and 24 hours after the exposure. One hundred female Sprague-Dawley rats were randomly divided into four groups. Group 1 included 30 rats received light exposure without any other treatment. Group 2 included 30 rats received light exposure with intraperitoneal injection of normal saline. Group 3 included 30 rats received light exposure with intraperitoneal injection of saturated hydrogen saline. And Group 4 included the other 10 rats which did not receive any treatment. The amount of intraperitoneal injection of saturated hydrogen saline and normal saline was calculated in the ratio of 1ml/100g of rat weight. Specimens were collected and processed by H-E staining, ultrastructure observation, biochemical measurement. Morphological changes were observed by light microscope and transmission electron microscope (TEM) and the retinal outer nuclear layer (ONL) thickness was measured by IPP 6.0, while the malondialdehyde (MDA) was measured by colorimetric determination at 532nm. RESULTS: Although the structure of retina in Group 1 and Group 2 was injured heavily, the injury in Group 3 was mild. The differences between Group 1 and Group 2 were not significant. Compared with the rats in Group 1 and Group 2, the ones in Group 3 had more clearly demarcated retina structure and more ordered cells by light microscope and TEM observation. The ONL thicknesses (400 times) of four groups at each time point except between Group 1 and Group 2 were significantly different (P<0.05). The thicknesses of the ONL in Group 1 at three time points were 30.41 +/- 4.04 mu m, 26.11 +/- 2.82 mu m and 20.63 +/- 1.06 mu m, in Group 2 were 31.62 +/- 4.54 mu m, 25.08 +/- 3.63 mu m and 19.07 +/- 3.86 mu m, in Group 3 were 29.75 +/- 3.62 mu m, 28.83 +/- 1.97 mu m and 27.61 +/- 1.83 mu m. In Group 4 the mean of the thickness was 37.35 +/- 1AIM: To explore the effect of saturated hydrogen saline on blue light-induced retinal damage in rats. METHODS: The retinal damage of rats was induced by blue light exposure for 6 hours and examined 8 hours, 16 hours and 24 hours after the exposure. One hundred female Sprague-Dawley rats were randomly divided into four groups. Group 1 included 30 rats received light exposure without any other treatment. Group 2 included 30 rats received light exposure with intraperitoneal injection of normal saline. Group 3 included 30 rats received light exposure with intraperitoneal injection of saturated hydrogen saline. And Group 4 included the other 10 rats which did not receive any treatment. The amount of intraperitoneal injection of saturated hydrogen saline and normal saline was calculated in the ratio of 1ml/100g of rat weight. Specimens were collected and processed by H-E staining, ultrastructure observation, biochemical measurement. Morphological changes were observed by light microscope and transmission electron microscope (TEM) and the retinal outer nuclear layer (ONL) thickness was measured by IPP 6.0, while the malondialdehyde (MDA) was measured by colorimetric determination at 532nm. RESULTS: Although the structure of retina in Group 1 and Group 2 was injured heavily, the injury in Group 3 was mild. The differences between Group 1 and Group 2 were not significant. Compared with the rats in Group 1 and Group 2, the ones in Group 3 had more clearly demarcated retina structure and more ordered cells by light microscope and TEM observation. The ONL thicknesses (400 times) of four groups at each time point except between Group 1 and Group 2 were significantly different (P<0.05). The thicknesses of the ONL in Group 1 at three time points were 30.41 +/- 4.04 mu m, 26.11 +/- 2.82 mu m and 20.63 +/- 1.06 mu m, in Group 2 were 31.62 +/- 4.54 mu m, 25.08 +/- 3.63 mu m and 19.07 +/- 3.86 mu m, in Group 3 were 29.75 +/- 3.62 mu m, 28.83 +/- 1.97 mu m and 27.61 +/- 1.83 mu m. In Group 4 the mean of the thickness was 37.35 +/- 1

关 键 词：RETINA HYDROGEN ANTIOXIDANTS PHOTOTOXICITY 

分 类 号：R774.1[医药卫生—眼科]