Metalloproteins/metalloenzymes for the synthesis of acetyl-CoA in the Wood-Ljungdahl pathway  

作　　者：ZHU XiaoFei1 & TAN XiangShi1,2 1 Department of Chemistry,Fudan University,Shanghai 200433,China 2 Institutes of Biomedical Sciences,Fudan University,Shanghai 200433,China 

出　　处：《Science China Chemistry》2009年第12期2071-2082,共12页中国科学（化学英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 20771029);Shanghai Pujiang Talent Project (Grant No. 08PJ14017);Shanghai Leading Academic Discipline Project (Grant No. B108)

摘　　要：This paper focuses on the group of metalloproteins/metalloenzymes in the acetyl-coenzyme A synthesis pathway of anaerobic microbes called Wood-Ljungdahl pathway,including formate dehydrogenase (FDH),corrinoid iron sulfur protein (CoFeSP),acetyl-CoA synthase (ACS) and CO dehydrogenase (CODH). FDH,a key metalloenzyme involved in the conversion of carbon dioxide to methyltetrahydrofolate,catalyzes the reversible oxidation of formate to carbon dioxide. CoFeSP,as a methyl group transformer,accepts the methyl group from CH3-H4 folate and then transfers it to ACS. CODH reversibly catalyzes the reduction of CO2 to CO and ACS functions for acetyl-coenzyme A synthesis through condensation of the methyl group,CO and coenzyme A,to finish the whole pathway. This paper introduces the structure,function and reaction mechanisms of these enzymes.This paper focuses on the group of metalloproteins/metalloenzymes in the acetyl-coenzyme A synthesis pathway of anaerobic microbes called Wood-Ljungdahl pathway, including formate dehydrogenase (FDH), corrinoid iron sulfur protein (CoFeSP), acetyl-CoA synthase (ACS) and CO dehydrogenase (CODH). FDH, a key metalloenzyme involved in the conversion of carbon dioxide to methyltetrahydrofolate, catalyzes the reversible oxidation of formate to carbon dioxide. CoFeSP, as a methyl group transformer, accepts the methyl group from CH3-H4 folate and then transfers it to ACS. CODH reversibly catalyzes the reduction of CO2 to CO and ACS functions for acetyl-coenzyme A synthesis through condensation of the methyl group, CO and coenzyme A, to finish the whole pathway. This paper introduces the structure, function and reaction mechanisms of these enzymes.

关 键 词：Wood-Ljungdahl PATHWAY formate DEHYDROGENASE corrinoid iron sulfur protein ACETYL-COA synthase CO DEHYDROGENASE 

分 类 号：O629.8[理学—有机化学]