Novel cyclen-based linear polymer as a high-affinity binding material for DNA condensation  

作　　者：XIANG YongZhe WANG Na ZHANG Ji LI Kun ZHANG ZhongWei LIN HongHui YU XiaoQi 

机构地区：[1]Key Laboratory of Green Chemistry and Technology of Ministry of Education,College of Chemistry,Sichuan University,Chengdu 610064,China [2]Key Laboratory of Bio-resources and Ecoenvironment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610064,China

出　　处：《Science China Chemistry》2009年第4期483-488,共6页中国科学（化学英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 20725206 and 20732004);Specialized Research Fund for the Doctoral Program of Higher Education;Scientific Fund of Sichuan Province for Outstanding Young Scientist

摘　　要：A novel cyclen-based linear polyamine (POGEC) was designed and synthesized from the reaction between 1,3-propanediol diglycidyl ether and 1,7-bis(diethoxyphosphory)-1,4,7,10-tetraazacyclod-odecane. High-affinity binding between POGEC and DNA was demonstrated by agarose gel electrophoresis and scanning electron microscopy (SEM). Moreover,the formed POGEC/DNA complex (termed polyplex) could be disassociated to release the free DNA through addition of the physiological concentration of NaCl solution. Fluorescence spectrum was used to measure the high-affinity binding and DNA condensation capability of POGEC. Circular dichroism (CD) spectrum indicates that the DNA conformation did not change after binding to POEGC.A novel cyclen-based linear polyamine (POGEC) was designed and synthesized from the reaction between 1,3-propanediol diglycidyl ether and 1,7-bis(diethoxyphosphory)-1,4,7,10-tetraazacyclod- odecane. High-affinity binding between POGEC and DNA was demonstrated by agarose gel electrophoresis and scanning electron microscopy (SEM). Moreover, the formed POGEC/DNA complex (termed polyplex) could be disassociated to release the free DNA through addition of the physiological concentration of NaCl solution. Fluorescence spectrum was used to measure the high-affinity binding and DNA condensation capability of POGEC. Circular dichroism (CD) spectrum indicates that the DNA conformation did not change after binding to POEGC.

关 键 词：CYCLEN (1 4 7 10-tetraazacyclododecane) POLYAMINE POLYPLEX DNA CONDENSATION 

分 类 号：Q523[生物学—生物化学]