Endogenous leptin fluctuates in hepatic ischemia/reperfusion injury and represents a potential therapeutic target  

作　　者：Ji Lin, Guang-Tao Yan, Hui Xue, Xiu-Hua Hao, Lu-Huan Wang, Research Laboratory of Biochemistry, Basic Medical Institute, Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, China Xiao-Ning Gao, Department of Hematology, Clinical Division of Internal Medicine, Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, China 

出　　处：《World Journal of Gastroenterology》2010年第43期5424-5434,共11页世界胃肠病学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China, No. 30670821;National Key Technology R&D Program, No. 2006BAF07B01;Special Funds for Key Program of Public Welfare of National Ministry of Science and Technology, No. 2002D1A40019;Nursery Fund of Chinese PLA General Hospital, No. 06MP83

摘　　要：AIM: To evaluate the role of leptin in the internal disorders during hepatic ischemia/reperfusion injury. METHODS: A rat model of 70% hepatic ischemia/reperfusion injury was established, with groups of shamoperation (Sham), 60 min ischemia/60 min reperfusion (I60'R60'), I60'R150', I60'R240' and I60'R360'. Serum leptin was detected by a self-produced radioimmunoassay; serum glucose, total anti-oxidation capacity, myeloperoxidase, alanine transaminase and diamine oxidase were determined by relevant kits, while histologicalalterations and protein levels of leptin in the lung, liver and duodenum were examined by hematoxylin-eosin staining and immunohistochemistry. Spearman's rank correlation between leptin and other variables or grading of tissue impairment were analyzed simultaneously. RESULTS: Serum leptin in I60'R360' was significantly higher than in Sham and I60'R240' groups (both P < 0.05), serum glucose in I60'R360' was higher than in Sham and I60'R150' (both P < 0.05), and serum total anti-oxidation capacity in I60'R240' and I60'R360' were higher than in Sham (both P < 0.05) and I60'R150' groups (both P < 0.01). Serum myeloperoxidase in groups of I60'R240' and I60'R360' were lower than in I60'R150'group (both P < 0.05), serum alanine transaminase in the four reperfusion groups were higher than in the Sham group (all P < 0.05), while serum DAO in I60'R360' was lower than in I60'R60' (P < 0.05). Histological impairment in the lung, liver and duodenum at the early phase of this injury was more serious, but the impairment at the later phase was lessened gradually. Protein levels of leptin in the lung in the four reperfusion groups were significantly lower than in the Sham group (all P < 0.01), decreasing in the order of I60'R150', I60' R60', I60'R360' and I60'R240'; the levels in the liver in I60'R60' and I60'R240' were higher than in the Sham group (both P < 0.01), while the levels in I60'R240' and I60'R360' were lower than in I60'R60' (both P < 0.01); the levels in duodenum in I60'R240' and I60'R360' were higherAIM: To evaluate the role of leptin in the internal disorders during hepatic ischemia/reperfusion injury. METHODS: A rat model of 70% hepatic ischemia/reperfusion injury was established, with groups of shamoperation (Sham), 60 min ischemia/60 min reperfusion (I60’R60’), I60’R150’, I60’R240’ and I60’R360’. Serum leptin was detected by a self-produced radioimmunoassay; serum glucose, total anti-oxidation capacity, myeloperoxidase, alanine transaminase and diamine oxidase were determined by relevant kits, while histologicalalterations and protein levels of leptin in the lung, liver and duodenum were examined by hematoxylin-eosin staining and immunohistochemistry. Spearman’s rank correlation between leptin and other variables or grading of tissue impairment were analyzed simultaneously. RESULTS: Serum leptin in I60’R360’ was significantly higher than in Sham and I60’R240’ groups (both P &lt; 0.05), serum glucose in I60’R360’ was higher than in Sham and I60’R150’ (both P &lt; 0.05), and serum total anti-oxidation capacity in I60’R240’ and I60’R360’ were higher than in Sham (both P &lt; 0.05) and I60’R150’ groups (both P &lt; 0.01). Serum myeloperoxidase in groups of I60’R240’ and I60’R360’ were lower than in I60’R150’group (both P &lt; 0.05), serum alanine transaminase in the four reperfusion groups were higher than in the Sham group (all P &lt; 0.05), while serum DAO in I60’R360’ was lower than in I60’R60’ (P &lt; 0.05). Histological impairment in the lung, liver and duodenum at the early phase of this injury was more serious, but the impairment at the later phase was lessened gradually. Protein levels of leptin in the lung in the four reperfusion groups were significantly lower than in the Sham group (all P &lt; 0.01), decreasing in the order of I60’R150’, I60’ R60’, I60’R360’ and I60’R240’; the levels in the liver in I60’R60’ and I60’R240’ were higher than in the Sham group (both P &lt; 0.01), while the levels in I60’R240’

关 键 词：LEPTIN REPERFUSION injury Liver LUNG DUODENUM Recovery of function 

分 类 号：R575[医药卫生—消化系统]