Recent developments of nanoparticle-based enrichment methods for mass spectrometric analysis in proteomics  

作　　者：ZHANG LiJuan, LU HaoJie & YANG PengYuan Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China 

出　　处：《Science China Chemistry》2010年第4期695-703,共9页中国科学（化学英文版）

基　　金：support from the Major State Basie Research Development Program (Grant No. 2007CB914100);the National Natural Science Foundation of China (Grant Nos. 20875016 & 20735005);the Ph.D. Programs Foundation of the Ministry of Education of China (Grant No. 200802460011);Shanghai Projects (Grant Nos. 08DZ2293601, Eastern Scholar, Shu Guang and B109)

摘　　要：In proteome research, rapid and effective separation strategies are essential for successful protein identification due to the broad dynamic range of proteins in biological samples. Some important proteins are often expressed in ultra low abundance, thus making the pre-concentration procedure before mass spectrometric analysis prerequisite. The main purpose of enrichment is to isolate target molecules from complex mixtures to reduce sample complexity and facilitate the subsequent analyzing steps. The introduction of nanoparticles into this field has accelerated the development of enrichment methods. In this review, we mainly focus on recent developments of using different nanomaterials for pre-concentration of low-abundance peptides/ proteins, including those containing post-translational modifications, such as phosphorylation and glycosylation, prior to mass spectrometric analysis.In proteome research, rapid and effective separation strategies are essential for successful protein identification due to the broad dynamic range of proteins in biological samples. Some important proteins are often expressed in ultra low abundance, thus making the pre-concentration procedure before mass spectrometric analysis prerequisite. The main purpose of enrichment is to isolate target molecules from complex mixtures to reduce sample complexity and facilitate the subsequent analyzing steps. The introduction of nanoparticles into this field has accelerated the development of enrichment methods. In this review, we mainly focus on recent developments of using different nanomaterials for pre-concentration of low-abundance peptides/ proteins, including those containing post-translational modifications, such as phosphorylation and glycosylation, prior to mass spectrometric analysis.

关 键 词：ENRICHMENT nanoparticles mass SPECTROMETRY PROTEOMICS PHOSPHORYLATION GLYCOSYLATION 

分 类 号：Q51[生物学—生物化学]