纤维素酶活力及混合纤维素酶协同作用的研究  

Cellulase activity and synergism of mixed cellulases.

作　　者：冯月[1] 蒋建新[1] 朱莉伟[1] 菅红磊[1] 

机构地区：[1]北京林业大学材料科学与技术学院,林业生物质材料与能源教育部工程研究中心,木质材料科学与应用教育部重点实验室

出　　处：《北京林业大学学报》2009年第S1期169-173,共5页Journal of Beijing Forestry University

基　　金：教育部新世纪优秀人才资助计划项目(NCET-07-0082);中国博士后科学基金项目(20060400401)

摘　　要：该文评价了4种商业纤维素酶的总酶活力、内切葡聚糖酶活力、外切葡聚糖酶活力及纤维二糖酶活力,并分析了不同来源的纤维素酶制剂混合后的酶活力的变化,以及表面活性剂对酶活力的影响。结果表明:纤维素酶复合酶系中的纤维二糖酶是影响整个纤维素酶活力的重要因素,且不同来源的纤维素酶中的内切、外切葡聚糖酶及纤维二糖酶之间存在同工酶的效应;加入的表面活性剂浓度不同,对各种酶制剂的酶活力影响不同。向其中两种混合后的纤维素酶中加入0.01%浓度的表面活性剂Tween 80,可以提高酶活力,促进纤维素的降解。Activity of endoglucanase,exoglucanase,cellobiase and filter paper enzyme of four kinds of commercial cellulases were evaluated.Sources of cellulases were mixed,and enzyme activities of these mixtures were then determined.The impact of surfactants on the enzyme activity was studied as well.Results showed that cellobiase was the key factor to the total complex cellulase system.Additionally, the endoglucanases,exoglucanases and cellobiases from different complex cellulase systems were able to act as isoenzymes.The effect of surfactant on the cellulase activity varied with the concentration of the surfactant.Surfactant Tween 80 was added to two of the mixed cellulases in a concentration of 0.01%,which increased the cellulase activity and hastened the degradation of cellulose.

关 键 词：纤维素酶 酶活评价 协同作用 表面活性剂 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]