Influence of carbonyl stress on rheological alterations of blood materials and decarbonylation effect of glutathione  

作　　者：彭密军 蔡建光 贺洪 龚萍 李国林 汤婷 朱泽瑞 印大中 

机构地区：[1]Key laboratory of Protein Chemistry and Developmental Biology of Ministry of Education,Hunan Normal University [2]College of Life Science,Hunan Normal University [3]Key laboratory of Hunan Forest Products and Chemical Engineering,Jishou University

出　　处：《Journal of Central South University》2008年第S1期540-544,共5页中南大学学报（英文版）

基　　金：Project(2007AA02Z433) supported by the National High Technology Research and Development Program of China;Project(2007RS4017) supported by the Postdoctoral Science Foundation of Hunan Province,China

摘　　要：The effects of various toxic carbonyls such as malondialdehyde(MDA),a secondary product of lipid peroxidation,and other aldehydes on rheological parameters and their relationship with aging-associated alterations were studied.Both MDA and glutaraldehyde(Glu) in different concentrations significantly increase viscosity,plastic viscosity and yield stress of human plasma and erythrocyte suspensions.MDA(20 mmol/L) reduces sharply the typical fluorescence of proteins(excitation 280 nm/emission 350 nm),and produces age pigment-like fluorescence with a strong emission peak at 460 nm when excites at 395 nm by only being incubated for some hours.In contrast,Glu decreases merely the fluorescence of proteins without producing age pigment-like fluorescence.These data suggest interestingly that the MDA-induced gradual protein cross linking seems to form from different mechanisms compared to the fast rheological changes of blood materials which may take place either in acute and chronic diseases or during aging.On the other hand,MDA induces various deleterious alterations of erythrocytes whereas glutathione(GSH) inhibits the MDA-related carbonyl stress in a concentration-dependent manner.The results indicate that carbonyl-amino reaction exists in the blood widely and GSH has the ability to interrupt or reverse this reaction in a certain way.It implies that carbonyl stress may be one of the important factors in blood stasis and suggests a theoretical and practical approach in anti-stresses and anti-aging.The effects of various toxic carbonyls such as malondialdehyde (MDA), a secondary product of lipid peroxidation, and other aldehydes on rheological parameters and their relationship with aging-associated alterations were studied. Both MDA and glutaraldehyde (Glu) in different concentrations significantly increase viscosity, plastic viscosity and yield stress of human plasma and erythrocyte suspensions. MDA (20 mmol/L) reduces sharply the typical fluorescence of proteins (excitation 280 nm/emission 350 nm), and produces age pigment-like fluorescence with a strong emission peak at 460 nm when excites at 395 nm by only being incubated for some hours. In contrast, Glu decreases merely the fluorescence of proteins without producing age pigment-like fluorescence. These data suggest interestingly that the MDA-induced gradual protein cross linking seems to form from different mechanisms compared to the fast rheological changes of blood materials which may take place either in acute and chronic diseases or during aging. On the other hand, MDA induces various deleterious alterations of erythrocytes whereas glutathione (GSH) inhibits the MDA-related carbonyl stress in a concentration-dependent manner. The results indicate that carbonyl-amino reaction exists in the blood widely and GSH has the ability to interrupt or reverse this reaction in a certain way. It implies that carbonyl stress may be one of the important factors in blood stasis and suggests a theoretical and practical approach in anti-stresses and anti-aging.

关 键 词：BLOOD viscosity CARBONYL stress MALONDIALDEHYDE cross linking DECARBONYLATION effect GLUTATHIONE 

分 类 号：R318.08[医药卫生—生物医学工程]