Protein kinase TTK interacts and co-localizes with CENP-E to the kinetochore of human cells  

作　　者：Jie Zhang Chuanhai Fu Yong Miao Zhen Dou Xuebiao Yao 

机构地区：[1]Univ Sci & Technol China, Lab Cell Dynam, Hefei 230027, Peoples R China

出　　处：《Chinese Science Bulletin》2002年第23期2005-2009,共5页

基　　金：This work was supported in part by the National Natural Science Foundation of China(Grant No.39925018);the Key Project of the Chinese Academy of Sciences(Grant No.KSCX2-2-01).

摘　　要：Spindle checkpoint is an important biochemical signaling cascade during mitosis which monitors the fidelity of chromosome segregation, and is mediated by protein kinases Mpsl and Bubl/BubRl. Our recent studies show that kinesin-related motor protein CENP-E interacts with BubRl and participates in spindle checkpoint signaling. To elucidate the molecular mechanisms underlying spindle checkpoint signaling, we carried out proteomic dissection of human cell kinetochore and revealed protein kinase TTK, human homologue of yeast Mpsl. Our studies show that TTK is localized to the kinetochore of human cells, and interacts with CENP-E, suggesting that TTK may play an important role in chromosome segregation during mitosis.Spindle checkpoint is an important biochemical signaling cascade during mitosis which monitors the fidelity of chromosome segregation, and is mediated by protein kinases Mps1 and Bub1/BubR1. Our recent studies show that kinesin-related motor protein CENP-E interacts with BubR1 and participates in spindle checkpoint signaling. To elucidate the molecular mechanisms underlying spindle checkpoint signaling, we carried out proteomic dissection of human cell kinetochore and revealed protein kinase TTK, human homologue of yeast Mps1. Our studies show that TTK is localized to the kinetochore of human cells, and interacts with CENP-E, suggesting that TTK may play an important role in chromosome segregation during mitosis.

关 键 词：SPINDLE CHECKPOINT TTK protein KINASE CENP-E kinetochore. 

分 类 号：Q987[生物学—遗传学]